Increased antibiotic resistance exhibited by the biofilm of Vibrio cholerae O139

Abstract Background Vibrio cholerae, the aetiological agent of the deadly diarrhoeal disease cholera, is known to form biofilm. The antibiotic susceptibility status of biofilm of V. cholerae O139, an important epidemic strain in India and other countries, has not previously been studied in detail. M...

Full description

Saved in:
Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2018-07, Vol.73 (7), p.1841-1847
Main Authors: Gupta, Preeti, Mankere, Bharti, Chekkoora Keloth, Shami, Tuteja, Urmil, Pandey, Pratibha, Chelvam, Kulanthaivel Thava
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background Vibrio cholerae, the aetiological agent of the deadly diarrhoeal disease cholera, is known to form biofilm. The antibiotic susceptibility status of biofilm of V. cholerae O139, an important epidemic strain in India and other countries, has not previously been studied in detail. Methods Antibiotic susceptibility status of planktonic and biofilm cultures of V. cholerae O139 was evaluated by determining MIC, MBC and minimum biofilm eradication concentration (MBEC) values of five different classes of antibiotics using established methods. Effects of antibiotic treatment on planktonic and biofilm cultures were analysed by scanning electron microscopy. The virulence of the antibiotic-surviving population (ASP) was evaluated using an infant mouse model. The frequency of spontaneous mutants and inheritability of antibiotic resistance were determined with standard methods. Results The antibiotic resistance exhibited by biofilm of V. cholerae O139 was found to be significantly higher (P 
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dky127