Potential Use of Random Amplified Polymorphic DNA Marker in Assessment of Genetic Diversity and Identification of Rapeseed (Brassica napus L.) Cultivars

• Published in: Biotechnology (Faisalābād, Pakistan) Pakistan), 2006-03, Vol.5 (2), p.153-159
• Main Authors: Shiran, Behrouz Shiran, ., Roghaieh Azimkhani, ., Shahram Mohammadi, ., Mohammad Reza Ahmadi
• Format: Article
• Language: English
• Subjects: Brassica napus; Brassica rapa
• ISSN: 1682-296X
• DOI: 10.3923/biotech.2006.153.159

In this study the genetic diversity among 27 rapeseed (B. napus) cultivars and one cultivar of B. rapa was investigated using Random Amplified Polymorphic DNA (RAPD) marker. A set of 24 arbitrary primers was used which produced 133 reliable polymorphic DNA bands ranging in size from 440 to 2988 bp. RAPD data were used to compute genetic similarities based on Jaccrad (J) and Simple Matching (SM) indexes. Similarity coefficients values ranged from 0.22 to 0.87 with a mean of 0.48 for J and from 0.13 to 0.61, with a mean of 0.31 for SM coefficient, for all genotypes. Similarity matrices were used to contrast dendrograms using Unweighted Paired Group of Arithmetic Mean (UPMGA) algorithm. In cluster analysis, based on both similarity coefficients, B. napus cultivars were grouped together and Hysyn110, which belongs to B. rapa was classified as an outlier. Winter and spring B. napus cultivars were clustered into two separate groups. Results of Principal Component Analysis (PCA) supported cluster analysis results strongly. RAPD profiling was found efficient to reveal DNA polymorphism among rapeseed cultivars. RAPD data showed relatively low level of genetic diversity among studied cultivars, but also revealed some genetically distinct cultivars. This finding should be useful to breeding programs for selection of the genetically divergent parents in order to obtain the highest level of heterosis.