Presence and function of kisspeptin/KISS1R system in swine ovarian follicles

• Published in: Theriogenology 2018-07, Vol.115, p.1-8
• Main Authors: Basini, G., Grasselli, F., Bussolati, S., Ciccimarra, R., Maranesi, M., Bufalari, A., Parillo, F., Zerani, M.
• Format: Article
• Language: English
• Subjects: Angiogenesis; Animals; Cell Proliferation - drug effects; Female; Granulosa cell; Granulosa Cells - chemistry; Granulosa Cells - drug effects; Granulosa Cells - physiology; KISS1R; Kisspeptin; Kisspeptins - analysis; Kisspeptins - antagonists & inhibitors; Kisspeptins - pharmacology; Kisspeptins - physiology; Neovascularization, Physiologic - drug effects; Ovarian Follicle - chemistry; Ovarian Follicle - physiology; Ovary - blood supply; Ovary - physiology; Oxidation-Reduction; Progesterone - biosynthesis; Receptors, Kisspeptin-1 - analysis; Receptors, Kisspeptin-1 - physiology; Steroids; Swine; VEGF
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2018.04.006

Kisspeptin and its receptor KISS1R are involved in the neuroendocrine regulation of mammalian reproduction and their role on follicular development and function can be hypothesized. The present work was designed to confirm the immunopresence of kisspeptin and its receptor in the ovary of swine and to study the effects of kisspeptin 10 and its antagonist, kisspeptin 234, on main functional parameters of granulosa cells (i.e. cell proliferation, steroid production, and redox status) as well as their modulatory action on angiogenesis. The immunopresence of kisspeptin and KISS1R were detected in granulosa cells. Kisspeptin 10 stimulated progesterone in vitro production, thus indirectly suggesting that it can have a role in the luteinization process of granulosa cells. Kisspeptin 10 displayed potentiating effects on non-enzymatic scavenging activity, thus supporting its involvement in the control of the antioxidant defense system of ovarian follicles. In addition, results from the angiogenesis bioassay suggest that kisspeptin may have a role in the physiological development of new ovarian vessels. Additional studies are needed to confirm the functional significance of the kisspeptin/KISS1R system within the swine ovary. •Kisspeptin and KISS1R are present in swine ovary granulosa and endothelial cells.•KP-10 and its antagonist KP-234 inhibit E2 and stimulate P4 granulosa cell output.•KP-234 stimulates NO production by granulosa cells.•KP-10 and KP-234 increase granulosa cell antioxidant power.•Endothelial cell growth is inhibited by KP-10 and KP-234.