Simultaneous determination of multi-residue and multi-class antibiotics in aquaculture shrimps by UPLC-MS/MS

• Published in: Food chemistry 2018-09, Vol.260, p.336-343
• Main Authors: Saxena, Sushil Kumar, Rangasamy, Rajesh, Krishnan, Anoop A., Singh, Dhirendra P., Uke, Sumedh P., Malekadi, Praveen Kumar, Sengar, Anoop S., Mohamed, D. Peer, Gupta, Ananda
• Format: Article
• Language: English
• Subjects: Antibiotics; Aquaculture products; Multi-class multi-residue; UPLC-MS/MS; Validation
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2018.04.018

•Single sample preparation, Single injection covering three groups of 24 antibiotics.•Method validation performance characteristics achieved as per CD 2002/657/EC.•Quantification approach using method matrix-match standard calibration curve.•Reliability of the method established by participation in proficiency testing.•Protocol applicable for regulatory monitoring of antibiotics in shrimp samples. An accurate, reliable and fast multi-residue, multi-class method using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was developed and validated for simultaneous determination and quantification of 24 pharmacologically active substances of three different classes (Quinolones including fluoroquinolones, sulphonamides and tetracyclines) in aquaculture shrimps. Sample preparation involves extraction with acetonitrile containing 0.1% formic acid and followed by clean up with n-hexane and 0.1% methanol in water by UPLC-MS/MS within 8 min. The method was validated according to European Commission Decision 2002/657. Acceptable values were obtained for linearity (5–200 μg kg−1), specificity, Limit of Quantification (5–10 μg kg−1), recovery (between 83 and 100%), repeatability (RSD