Screening and analysis of key active constituents in Guanxinshutong capsule using mass spectrum and integrative network pharmacology

Guanxinshutong capsule (GXSTC) is an effective and safe traditional Chinese medicine used in the treatment of cardiovascular diseases (CVDs) for many years. However, the targets of this herbal formula and the underlying molecular mechanisms of action involved in the treatment of CVDs are still uncle...

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Bibliographic Details
Published in:Chinese journal of natural medicines 2018-04, Vol.16 (4), p.302-312
Main Authors: LIU, Feng, DU, Xia, LIU, Pei-Rong, SUN, Yu-Hong, ZHANG, Yan-Min
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Bornanes - chemistry
Calcium signaling pathway
Cardiotonic Agents - chemistry
Cardiotonic Agents - pharmacology
Cardiovascular diseases
Cells, Cultured
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - pharmacology
Eugenol - chemistry
Gene Expression - drug effects
Guanxinshutong capsule
Hydroxybenzoates - chemistry
Mass Spectrometry
Mass spectrum
Models, Biological
Myocytes, Cardiac - drug effects
Nitric Oxide Synthase Type III - genetics
Phenanthrenes - chemistry
Rats
Rats, Sprague-Dawley
Receptor, PAR-1 - genetics
Systems Biology
Systems pharmacology
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Summary:Guanxinshutong capsule (GXSTC) is an effective and safe traditional Chinese medicine used in the treatment of cardiovascular diseases (CVDs) for many years. However, the targets of this herbal formula and the underlying molecular mechanisms of action involved in the treatment of CVDs are still unclear. In the present study, we used a systems pharmacology approach to identify the active ingredients of GXSTC and their corresponding targets in the calcium signaling pathway with respect to the treatment of CVDs. This method integrated chromatographic techniques, prediction of absorption, distribution, metabolism, and excretion, analysis using Kyoto Encyclopedia of Genes and Genomes, network construction, and pharmacological experiments. 12 active compounds and 33 targets were found to have a role in the treatment of CVDs, and four main active ingredients, including protocatechuic acid, cryptotanshinone, eugenol, and borneol were selected to verify the effect of (GXSTC) on calcium signaling system in cardiomyocyte injury induced by hypoxia and reoxygenation. The results from the present study revealed the active components and targets of GXSTC in the treatment of CVDs, providing a new perspective to enhance the understanding of the role of the calcium signaling pathway in the therapeutic effect of GXSTC.
ISSN:1875-5364
1875-5364
DOI:10.1016/S1875-5364(18)30060-8