FaptaSyme: A Strategy for Converting a Monomer/Oligomer‐Nonselective Aptameric Sensor into an Oligomer‐Selective One

Aptameric sensors can bind molecular targets and produce output signals, a phenomenon that is used in bioassays. In some cases, it is important to distinguish between monomeric and oligomeric forms of a target. Here, we propose a strategy to convert a monomer/oligomer‐nonselective sensor into an oli...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2018-06, Vol.19 (11), p.1123-1126
Main Authors: Evangelista, Baggio A., Kim, Yoon‐Seong, Kolpashchikov, Dmitry M.
Format: Article
Language:English
Subjects:
aptamers
aptazymes
Bioassays
biosensors
deoxyribozymes
Fluorescence
Molecular chains
Monomers
Movement disorders
Neurodegenerative diseases
Neurological diseases
Parkinson's disease
Sensors
split probes
Strategy
Synuclein
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Summary:Aptameric sensors can bind molecular targets and produce output signals, a phenomenon that is used in bioassays. In some cases, it is important to distinguish between monomeric and oligomeric forms of a target. Here, we propose a strategy to convert a monomer/oligomer‐nonselective sensor into an oligomer‐selective sensor. We designed an aptazyme that produced a high fluorescent output in the presence of oligomeric α‐synuclein (a molecular marker of Parkinson's disease) but not its monomeric form. The strategy is potentially useful in the design of point‐of‐care tests for the diagnosis of neurodegenerative diseases. The core of it all: Each DNA strand a and b consists of a full α‐synuclein (α‐syn)‐binding aptamer and one‐half of the catalytic core of an RNA‐cleaving deoxyribozyme that is able to report fluorescently only the oligomeric form of α‐syn but not its monomeric form.
ISSN:1439-4227
1439-7633
1439-7633
DOI:10.1002/cbic.201800017