The Enterococcus faecalis MSCRAMM ACE Binds Its Ligand by the Collagen Hug Model

• Published in: The Journal of biological chemistry 2007-07, Vol.282 (27), p.19629-19637
• Main Authors: Liu, Qing, Ponnuraj, Karthe, Xu, Yi, Ganesh, Vannakambadi K., Sillanpää, Jouko, Murray, Barbara E., Narayana, Sthanam V.L., Höök, Magnus
• Format: Article
• Language: English
• Subjects: Adhesins, Bacterial - chemistry; Adhesins, Bacterial - genetics; Adhesins, Bacterial - metabolism; Amino Acid Substitution; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Collagen - chemistry; Collagen - metabolism; Crystallography, X-Ray; Disulfides; Enterococcus faecalis; Enterococcus faecalis - chemistry; Enterococcus faecalis - genetics; Ligands; Models, Molecular; Mutation, Missense; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; Staphylococcus aureus
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M611137200

We have determined the crystal structure of the ligand binding segment of the Enterococcus faecalis collagen binding MSCRAMM ACE (microbial surface components recognizing adhesive matrix molecules adhesin of collagen from enterococci). This segment is composed of two subdomains, N1 and N2, each adopting an IgG-like fold and forming a putative collagen binding surface at the interface between the two subdomains. This structure is very similar to that recently reported for CNA, the collagen binding MSCRAMM of Staphylococcus aureus, for which a unique ligand binding mechanism called the Collagen Hug was proposed. We suggest that ACE binds collagen by a similar mechanism and present the first biochemical evidence for this binding model. Replacing residues in the putative collagen binding trench of ACE N2 with Ala residues affected collagen binding. A closed conformation of ACE stabilized by an engineered disulfide bond is unable to bind collagen. Finally, the importance of the residues in the N2 extension in stabilizing the MSCRAMM-ligand complex is demonstrated by selected point and truncation mutations.