Synthesis, biodegradability and cytotoxicity of water-soluble isobutylchitosan

The objective of the present study was to improve the solubility of chitosan at neutral aqueous solution without altering its bioactivity. Through an alkylation reaction under mild conditions, isobutyl was introduced to chitosan to form isobutylchitosan, which showed a good solubility in neutral aqu...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate polymers 2007-01, Vol.67 (1), p.40-45
Main Authors: Li, Dong-Hong, Liu, Liang-Ming, Tian, Kun-Lun, Liu, Jian-Chang, Fan, Xiao-Qing
Format: Article
Language:English
Subjects:
Applied sciences
Biodegradability
Cytotoxicity
Exact sciences and technology
Isobutylchitosan
Natural polymers
Physicochemistry of polymers
Starch and polysaccharides
Water solubility
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of the present study was to improve the solubility of chitosan at neutral aqueous solution without altering its bioactivity. Through an alkylation reaction under mild conditions, isobutyl was introduced to chitosan to form isobutylchitosan, which showed a good solubility in neutral aqueous media resulting from the reduced crystallinity of the product. The structure of isobutylchitosan was characterized by IR, 1H NMR spectroscopy, and X-ray diffraction. The degradation of the product was evaluated in vivo using a mouse model, and the cytotoxicity of the product in L929 mouse fibroblasts was monitored using the MTT assay and the release of the cytosolic enzyme lactate dehydrogenase (LDH). It was found that the product exhibited the properties of improved biodegradability, increased MTT values and decreased LDH release. The results suggested that isobutylchitosan might be one of promising and safe biomaterial with potential applications.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2006.04.022