Detection of microfilariae with counting chamber technique in some Nigerian rural communities

The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella per...

Full description

Saved in:
Bibliographic Details
Published in:African journal of biotechnology 2005-04, Vol.4 (4), p.367-370
Main Authors: Agbolade, OM, Akinboye, DO
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 370
container_issue 4
container_start_page 367
container_title African journal of biotechnology
container_volume 4
creator Agbolade, OM
Akinboye, DO
description The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella perstans are endemic. Blood samples were collected by finger-pricking method from 612 subjects (334 males, 278 females), between February 1996 and July 1998. Each blood sample was examined for mf using TS or/and CC technique(s). CC technique had statistically higher sensitivity (79.3%) than TS technique (39.1%). For both techniques, the microfilarial range was 1 to 20 mf/50 mu 1 of blood. There was no statistically significant differences between the microfilarial geometric mean intensities with both techniques.
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_20330642</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20330642</sourcerecordid><originalsourceid>FETCH-LOGICAL-p186t-892a8c39cee8eae5d552272bbb665819a11e40ae06a354448be75d887fbb5a8d3</originalsourceid><addsrcrecordid>eNpNjstOwzAURC0EEqXwD16xi-R33CUqT6mCDSxRde3cNEaJXWxH_D6RYNHVnMWc0ZyRFTdWNVpyfX7Cl-SqlC_GhBSKrcjnPVb0NaRIU0-n4HPqwwg5ANKfUAfq0xxriAfqB5gcZrrUhxi-Z6Qh0pImpK_hgIsQaZ4zjIsxTXMMNWC5Jhc9jAVv_nNNPh4f3rfPze7t6WV7t2uO3Jra2I0A6-XGI1oE1J3WQrTCOWeMtnwDnKNigMyA1Eop67DVnbVt75wG28k1uf3bPea0PCt1P4XicRwhYprLXjApmVFC_gKaOFOL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20330642</pqid></control><display><type>article</type><title>Detection of microfilariae with counting chamber technique in some Nigerian rural communities</title><source>Free Full-Text Journals in Chemistry</source><creator>Agbolade, OM ; Akinboye, DO</creator><creatorcontrib>Agbolade, OM ; Akinboye, DO</creatorcontrib><description>The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella perstans are endemic. Blood samples were collected by finger-pricking method from 612 subjects (334 males, 278 females), between February 1996 and July 1998. Each blood sample was examined for mf using TS or/and CC technique(s). CC technique had statistically higher sensitivity (79.3%) than TS technique (39.1%). For both techniques, the microfilarial range was 1 to 20 mf/50 mu 1 of blood. There was no statistically significant differences between the microfilarial geometric mean intensities with both techniques.</description><identifier>ISSN: 1684-5315</identifier><identifier>EISSN: 1684-5315</identifier><language>eng</language><ispartof>African journal of biotechnology, 2005-04, Vol.4 (4), p.367-370</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Agbolade, OM</creatorcontrib><creatorcontrib>Akinboye, DO</creatorcontrib><title>Detection of microfilariae with counting chamber technique in some Nigerian rural communities</title><title>African journal of biotechnology</title><description>The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella perstans are endemic. Blood samples were collected by finger-pricking method from 612 subjects (334 males, 278 females), between February 1996 and July 1998. Each blood sample was examined for mf using TS or/and CC technique(s). CC technique had statistically higher sensitivity (79.3%) than TS technique (39.1%). For both techniques, the microfilarial range was 1 to 20 mf/50 mu 1 of blood. There was no statistically significant differences between the microfilarial geometric mean intensities with both techniques.</description><issn>1684-5315</issn><issn>1684-5315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpNjstOwzAURC0EEqXwD16xi-R33CUqT6mCDSxRde3cNEaJXWxH_D6RYNHVnMWc0ZyRFTdWNVpyfX7Cl-SqlC_GhBSKrcjnPVb0NaRIU0-n4HPqwwg5ANKfUAfq0xxriAfqB5gcZrrUhxi-Z6Qh0pImpK_hgIsQaZ4zjIsxTXMMNWC5Jhc9jAVv_nNNPh4f3rfPze7t6WV7t2uO3Jra2I0A6-XGI1oE1J3WQrTCOWeMtnwDnKNigMyA1Eop67DVnbVt75wG28k1uf3bPea0PCt1P4XicRwhYprLXjApmVFC_gKaOFOL</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Agbolade, OM</creator><creator>Akinboye, DO</creator><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050401</creationdate><title>Detection of microfilariae with counting chamber technique in some Nigerian rural communities</title><author>Agbolade, OM ; Akinboye, DO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p186t-892a8c39cee8eae5d552272bbb665819a11e40ae06a354448be75d887fbb5a8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Agbolade, OM</creatorcontrib><creatorcontrib>Akinboye, DO</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>African journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agbolade, OM</au><au>Akinboye, DO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of microfilariae with counting chamber technique in some Nigerian rural communities</atitle><jtitle>African journal of biotechnology</jtitle><date>2005-04-01</date><risdate>2005</risdate><volume>4</volume><issue>4</issue><spage>367</spage><epage>370</epage><pages>367-370</pages><issn>1684-5315</issn><eissn>1684-5315</eissn><abstract>The conventional stained thick smear technique (TS) has innate possibility of underestimation of microfilariae (mf) in microfilaraemic subjects. The usability of counting chamber (CC) technique in the detection of mf was determined in three Nigerian rural communities where Loa loa and Mansonella perstans are endemic. Blood samples were collected by finger-pricking method from 612 subjects (334 males, 278 females), between February 1996 and July 1998. Each blood sample was examined for mf using TS or/and CC technique(s). CC technique had statistically higher sensitivity (79.3%) than TS technique (39.1%). For both techniques, the microfilarial range was 1 to 20 mf/50 mu 1 of blood. There was no statistically significant differences between the microfilarial geometric mean intensities with both techniques.</abstract><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1684-5315
ispartof African journal of biotechnology, 2005-04, Vol.4 (4), p.367-370
issn 1684-5315
1684-5315
language eng
recordid cdi_proquest_miscellaneous_20330642
source Free Full-Text Journals in Chemistry
title Detection of microfilariae with counting chamber technique in some Nigerian rural communities
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T20%3A36%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20microfilariae%20with%20counting%20chamber%20technique%20in%20some%20Nigerian%20rural%20communities&rft.jtitle=African%20journal%20of%20biotechnology&rft.au=Agbolade,%20OM&rft.date=2005-04-01&rft.volume=4&rft.issue=4&rft.spage=367&rft.epage=370&rft.pages=367-370&rft.issn=1684-5315&rft.eissn=1684-5315&rft_id=info:doi/&rft_dat=%3Cproquest%3E20330642%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p186t-892a8c39cee8eae5d552272bbb665819a11e40ae06a354448be75d887fbb5a8d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20330642&rft_id=info:pmid/&rfr_iscdi=true