Mid-Membrane Photolabeling of the Transmembrane Domain of Glycophorin A in Phospholipid Vesicles

The tandem use of the photosensitive bola‐amphiphile 1 (X=3H) and cholesterol enabled the determination of the center of the transmembrane domain of glycophorin A (131 amino acid residues) in a membrane by selective functionalization of the protein within a phospholipid bilayer.

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2001-03, Vol.40 (5), p.944-946
Main Authors: Ogawa, Yoshikatsu, Hahn, Wolfgang, Garnier, Philippe, Higashi, Nobuaki, Massotte, Dominique, Metz-Boutigue, Marie-Hélène, Rousseau, Bernard, Sunamoto, Junzo, Ourisson, Guy, Nakatani, Yoichi
Format: Article
Language:English
Subjects:
cross-coupling
liposomes
membrane proteins
photolabeling
protein structures
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Description
Summary:The tandem use of the photosensitive bola‐amphiphile 1 (X=3H) and cholesterol enabled the determination of the center of the transmembrane domain of glycophorin A (131 amino acid residues) in a membrane by selective functionalization of the protein within a phospholipid bilayer.
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20010302)40:5<944::AID-ANIE944>3.0.CO;2-O