Mass Spectrometry of an Intact Virus

The simultaneous measurement of the time‐of‐flight and the number of charges on each electrosprayed ion provides a new way to determine the mass of a whole intact virus. The weights of an icosahedral virus (rice yellow mottle virus) consisting of a single‐stranded RNA surrounded by a homogeneous pro...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2001-02, Vol.40 (3), p.541-544
Main Authors: Fuerstenau, Stephen D., Benner, W. Henry, Thomas, John J., Brugidou, Christophe, Bothner, Brian, Siuzdak, Gary
Format: Article
Language:English
Subjects:
mass spectrometry
noncovalent interactions
RNA structures
viruses
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Summary:The simultaneous measurement of the time‐of‐flight and the number of charges on each electrosprayed ion provides a new way to determine the mass of a whole intact virus. The weights of an icosahedral virus (rice yellow mottle virus) consisting of a single‐stranded RNA surrounded by a homogeneous protein shell with a mass of 6.5×106 Da, and a rod‐shaped RNA virus (tobacco mosaic virus) with a mass of 40.5×106 Da were measured with this technique.
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20010202)40:3<541::AID-ANIE541>3.0.CO;2-K