How to calculate thermostability of enzymes using a simple approach

Determination of thermostability of enzymes is of prime importance for their successful industrial applications and, yet, the published data has often been incompletely analyzed to assess the suitability of enzymes. It is possible to determine meaningful thermostability parameters from the routinely...

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Bibliographic Details
Published in:Biochemistry and molecular biology education 2018-07, Vol.46 (4), p.398-402
Main Authors: Saqib, Abdul A. N., Siddiqui, Khawar S.
Format: Article
Language:English
Subjects:
activation thermodynamics
Biochemistry
biotechnology education
College Science
Computation
Enzymes
enzymology
Industrial applications
Laboratory Equipment
Molecular Biology
Science Instruction
Science Laboratories
Scientific Concepts
Teaching Methods
Thermal stability
Thermodynamics
thermostability
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Summary:Determination of thermostability of enzymes is of prime importance for their successful industrial applications and, yet, the published data has often been incompletely analyzed to assess the suitability of enzymes. It is possible to determine meaningful thermostability parameters from the routinely acquired data through a straightforward method that is not only more informative but also provides a means to compare thermostability of enzymes from different sources. Here, we describe a simple, effective, and economical way to determine enzyme thermostability. In our opinion, including this method in Biochemistry and Molecular Biology curricula will encourage students to include thermostability analysis in their future work, leading to a more meaningful approach to evaluate and compare enzymes. Furthermore, as the method requires minimum specialized equipment, the analysis will be particularly suitable for labs that cannot afford expensive setup. © 2018 by The International Union of Biochemistry and Molecular Biology, 46:398–402, 2018.
ISSN:1470-8175
1539-3429
DOI:10.1002/bmb.21127