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Multivariate Analysis of Increase in Life Span of Caenorhabditis elegans Through Intestinal Colonization by Indigenous Probiotic Strains
The present study aimed to analyze the colonization potential of indigenous probiotic strains and to assess their effects on physiology of Caenorhabditis elegans. The protective effect of probiotics was evaluated in terms of increase in life span of the worm through colonization in the intestine. A...
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| Published in: | Probiotics and antimicrobial proteins 2019-09, Vol.11 (3), p.865-873 |
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| creator | Sharma, Kavita Pooranachithra, Murugesan Balamurugan, Krishnaswamy Goel, Gunjan |
| description | The present study aimed to analyze the colonization potential of indigenous probiotic strains and to assess their effects on physiology of
Caenorhabditis elegans.
The protective effect of probiotics was evaluated in terms of increase in life span of the worm through colonization in the intestine. A total of 15 probiotic cultures were evaluated for their effect on mean life span, pharyngeal pumping, and normal reproduction behavior in the worms. The chemotactic behavior in terms of binary choice index was also evaluated. The adherence and colonization of the intestine of the worm by probiotics were monitored at different time intervals by enumerating the microbial population and fluorescent microscopic observations. The survival analysis-based Kaplan–Meier method indicated that the probiotic cultures increased the survival probability as compared to control strain
E. coli
OP50. There was no effect of feeding probiotics on physiological responses of the worm such as pharyngeal pumping and reproduction. The principal component analysis (PCA) of the results indicated
Lactobacillus plantarum
K90 and
L. paracasei
CD4 as potential probiotics with binary choice index of 0.8 as food preference of
C. elegans
. The strains exhibit higher adherence and colonization in the gut of worms and increased the life span by 5 days as compared to control
E. coli
OP50. In conclusion, feeding with probiotic cultures is effective in extending the lifespan of
C. elegans
; however, the colonization ability differs among the strains. |
| doi_str_mv | 10.1007/s12602-018-9420-0 |
| format | article |
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Caenorhabditis elegans.
The protective effect of probiotics was evaluated in terms of increase in life span of the worm through colonization in the intestine. A total of 15 probiotic cultures were evaluated for their effect on mean life span, pharyngeal pumping, and normal reproduction behavior in the worms. The chemotactic behavior in terms of binary choice index was also evaluated. The adherence and colonization of the intestine of the worm by probiotics were monitored at different time intervals by enumerating the microbial population and fluorescent microscopic observations. The survival analysis-based Kaplan–Meier method indicated that the probiotic cultures increased the survival probability as compared to control strain
E. coli
OP50. There was no effect of feeding probiotics on physiological responses of the worm such as pharyngeal pumping and reproduction. The principal component analysis (PCA) of the results indicated
Lactobacillus plantarum
K90 and
L. paracasei
CD4 as potential probiotics with binary choice index of 0.8 as food preference of
C. elegans
. The strains exhibit higher adherence and colonization in the gut of worms and increased the life span by 5 days as compared to control
E. coli
OP50. In conclusion, feeding with probiotic cultures is effective in extending the lifespan of
C. elegans
; however, the colonization ability differs among the strains.</description><identifier>ISSN: 1867-1306</identifier><identifier>EISSN: 1867-1314</identifier><identifier>DOI: 10.1007/s12602-018-9420-0</identifier><identifier>PMID: 29717419</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Applied Microbiology ; Bacterial Adhesion - physiology ; Caenorhabditis elegans ; Caenorhabditis elegans - drug effects ; Caenorhabditis elegans - growth & development ; Caenorhabditis elegans - microbiology ; CD4 antigen ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Colonization ; E coli ; Escherichia coli - growth & development ; Feeding ; Female ; Food preferences ; Intestine ; Intestines - microbiology ; Lactobacillus paracasei - growth & development ; Lactobacillus paracasei - physiology ; Lactobacillus plantarum - growth & development ; Lactobacillus plantarum - physiology ; Life span ; Longevity - drug effects ; Male ; Microbiology ; Multivariate Analysis ; Nematodes ; Nutrition ; Pharynx ; Principal components analysis ; Probiotics ; Probiotics - pharmacology ; Protein Science ; Survival ; Survival analysis ; Worms</subject><ispartof>Probiotics and antimicrobial proteins, 2019-09, Vol.11 (3), p.865-873</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Probiotics and Antimicrobial Proteins is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-c96e0a17c2477cf13b6c1d79ab3ae3daca8abc56b70331af7f697843d8b37f353</citedby><cites>FETCH-LOGICAL-c372t-c96e0a17c2477cf13b6c1d79ab3ae3daca8abc56b70331af7f697843d8b37f353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29717419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Kavita</creatorcontrib><creatorcontrib>Pooranachithra, Murugesan</creatorcontrib><creatorcontrib>Balamurugan, Krishnaswamy</creatorcontrib><creatorcontrib>Goel, Gunjan</creatorcontrib><title>Multivariate Analysis of Increase in Life Span of Caenorhabditis elegans Through Intestinal Colonization by Indigenous Probiotic Strains</title><title>Probiotics and antimicrobial proteins</title><addtitle>Probiotics & Antimicro. Prot</addtitle><addtitle>Probiotics Antimicrob Proteins</addtitle><description>The present study aimed to analyze the colonization potential of indigenous probiotic strains and to assess their effects on physiology of
Caenorhabditis elegans.
The protective effect of probiotics was evaluated in terms of increase in life span of the worm through colonization in the intestine. A total of 15 probiotic cultures were evaluated for their effect on mean life span, pharyngeal pumping, and normal reproduction behavior in the worms. The chemotactic behavior in terms of binary choice index was also evaluated. The adherence and colonization of the intestine of the worm by probiotics were monitored at different time intervals by enumerating the microbial population and fluorescent microscopic observations. The survival analysis-based Kaplan–Meier method indicated that the probiotic cultures increased the survival probability as compared to control strain
E. coli
OP50. There was no effect of feeding probiotics on physiological responses of the worm such as pharyngeal pumping and reproduction. The principal component analysis (PCA) of the results indicated
Lactobacillus plantarum
K90 and
L. paracasei
CD4 as potential probiotics with binary choice index of 0.8 as food preference of
C. elegans
. The strains exhibit higher adherence and colonization in the gut of worms and increased the life span by 5 days as compared to control
E. coli
OP50. In conclusion, feeding with probiotic cultures is effective in extending the lifespan of
C. elegans
; however, the colonization ability differs among the strains.</description><subject>Animals</subject><subject>Applied Microbiology</subject><subject>Bacterial Adhesion - physiology</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - drug effects</subject><subject>Caenorhabditis elegans - growth & development</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>CD4 antigen</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Colonization</subject><subject>E coli</subject><subject>Escherichia coli - growth & development</subject><subject>Feeding</subject><subject>Female</subject><subject>Food preferences</subject><subject>Intestine</subject><subject>Intestines - microbiology</subject><subject>Lactobacillus paracasei - growth & development</subject><subject>Lactobacillus paracasei - physiology</subject><subject>Lactobacillus plantarum - growth & development</subject><subject>Lactobacillus plantarum - physiology</subject><subject>Life span</subject><subject>Longevity - drug effects</subject><subject>Male</subject><subject>Microbiology</subject><subject>Multivariate Analysis</subject><subject>Nematodes</subject><subject>Nutrition</subject><subject>Pharynx</subject><subject>Principal components analysis</subject><subject>Probiotics</subject><subject>Probiotics - pharmacology</subject><subject>Protein Science</subject><subject>Survival</subject><subject>Survival analysis</subject><subject>Worms</subject><issn>1867-1306</issn><issn>1867-1314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kc1qGzEUhUVpaFynD9BNEXTTzST6GUszy2Ca1OCQgJO1uNJobIWx5EqagvMEfezIOEmhkNUVnO-ce8VB6Csl55QQeZEoE4RVhDZVWzNSkQ9oQhshK8pp_fHtTcQp-pzSIyFCcEY-oVPWSipr2k7Q35txyO4PRAfZ4ksPwz65hEOPF95EC8li5_HS9RavduAPwhysD3EDunO5oHawa_AJ329iGNeb4ss2ZVeS8DwMwbsnyC54rPdF6ty6mMeE72LQLmRn8CpHcD6doZMehmS_vMwperj6eT__VS1vrxfzy2VluGS5Mq2wBKg0rJbS9JRrYWgnW9AcLO_AQAPazISWhHMKvexFK5uad43msuczPkU_jrm7GH6P5VK1dcnYYQBvy2GKFR9vaCvrgn7_D30MYywfKxSTXMzIjMtC0SNlYkgp2l7tottC3CtK1KEmdaxJlZrUoSZFiufbS_Kot7Z7c7z2UgB2BFKR_NrGf6vfT30GsnKfNg</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Sharma, Kavita</creator><creator>Pooranachithra, Murugesan</creator><creator>Balamurugan, Krishnaswamy</creator><creator>Goel, Gunjan</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20190901</creationdate><title>Multivariate Analysis of Increase in Life Span of Caenorhabditis elegans Through Intestinal Colonization by Indigenous Probiotic Strains</title><author>Sharma, Kavita ; Pooranachithra, Murugesan ; Balamurugan, Krishnaswamy ; Goel, Gunjan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-c96e0a17c2477cf13b6c1d79ab3ae3daca8abc56b70331af7f697843d8b37f353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Applied Microbiology</topic><topic>Bacterial Adhesion - physiology</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - drug effects</topic><topic>Caenorhabditis elegans - growth & development</topic><topic>Caenorhabditis elegans - microbiology</topic><topic>CD4 antigen</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Colonization</topic><topic>E coli</topic><topic>Escherichia coli - growth & development</topic><topic>Feeding</topic><topic>Female</topic><topic>Food preferences</topic><topic>Intestine</topic><topic>Intestines - microbiology</topic><topic>Lactobacillus paracasei - growth & development</topic><topic>Lactobacillus paracasei - physiology</topic><topic>Lactobacillus plantarum - growth & development</topic><topic>Lactobacillus plantarum - physiology</topic><topic>Life span</topic><topic>Longevity - drug effects</topic><topic>Male</topic><topic>Microbiology</topic><topic>Multivariate Analysis</topic><topic>Nematodes</topic><topic>Nutrition</topic><topic>Pharynx</topic><topic>Principal components analysis</topic><topic>Probiotics</topic><topic>Probiotics - pharmacology</topic><topic>Protein Science</topic><topic>Survival</topic><topic>Survival analysis</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Kavita</creatorcontrib><creatorcontrib>Pooranachithra, Murugesan</creatorcontrib><creatorcontrib>Balamurugan, Krishnaswamy</creatorcontrib><creatorcontrib>Goel, Gunjan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Probiotics and antimicrobial proteins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Kavita</au><au>Pooranachithra, Murugesan</au><au>Balamurugan, Krishnaswamy</au><au>Goel, Gunjan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multivariate Analysis of Increase in Life Span of Caenorhabditis elegans Through Intestinal Colonization by Indigenous Probiotic Strains</atitle><jtitle>Probiotics and antimicrobial proteins</jtitle><stitle>Probiotics & Antimicro. Prot</stitle><addtitle>Probiotics Antimicrob Proteins</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>11</volume><issue>3</issue><spage>865</spage><epage>873</epage><pages>865-873</pages><issn>1867-1306</issn><eissn>1867-1314</eissn><abstract>The present study aimed to analyze the colonization potential of indigenous probiotic strains and to assess their effects on physiology of
Caenorhabditis elegans.
The protective effect of probiotics was evaluated in terms of increase in life span of the worm through colonization in the intestine. A total of 15 probiotic cultures were evaluated for their effect on mean life span, pharyngeal pumping, and normal reproduction behavior in the worms. The chemotactic behavior in terms of binary choice index was also evaluated. The adherence and colonization of the intestine of the worm by probiotics were monitored at different time intervals by enumerating the microbial population and fluorescent microscopic observations. The survival analysis-based Kaplan–Meier method indicated that the probiotic cultures increased the survival probability as compared to control strain
E. coli
OP50. There was no effect of feeding probiotics on physiological responses of the worm such as pharyngeal pumping and reproduction. The principal component analysis (PCA) of the results indicated
Lactobacillus plantarum
K90 and
L. paracasei
CD4 as potential probiotics with binary choice index of 0.8 as food preference of
C. elegans
. The strains exhibit higher adherence and colonization in the gut of worms and increased the life span by 5 days as compared to control
E. coli
OP50. In conclusion, feeding with probiotic cultures is effective in extending the lifespan of
C. elegans
; however, the colonization ability differs among the strains.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>29717419</pmid><doi>10.1007/s12602-018-9420-0</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Applied Microbiology Bacterial Adhesion - physiology Caenorhabditis elegans Caenorhabditis elegans - drug effects Caenorhabditis elegans - growth & development Caenorhabditis elegans - microbiology CD4 antigen Chemistry Chemistry and Materials Science Chemistry/Food Science Colonization E coli Escherichia coli - growth & development Feeding Female Food preferences Intestine Intestines - microbiology Lactobacillus paracasei - growth & development Lactobacillus paracasei - physiology Lactobacillus plantarum - growth & development Lactobacillus plantarum - physiology Life span Longevity - drug effects Male Microbiology Multivariate Analysis Nematodes Nutrition Pharynx Principal components analysis Probiotics Probiotics - pharmacology Protein Science Survival Survival analysis Worms |
| title | Multivariate Analysis of Increase in Life Span of Caenorhabditis elegans Through Intestinal Colonization by Indigenous Probiotic Strains |
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