Serological, intradermal and live flea challenge tests in the assessment of hypersensitivity to flea antigens in cats (Felis domesticus)

• Published in: Parasitology research (1987) 2006-09, Vol.99 (4), p.392-397
• Main Authors: BOND, Ross, HUTCHINSON, Melanie J, LOEFFLER, Anette
• Format: Article
• Language: English
• Subjects: Animals; Antigens - immunology; Biological and medical sciences; Cat Diseases - blood; Cat Diseases - diagnosis; Cats; Dermatitis, Allergic Contact - diagnosis; Dermatitis, Allergic Contact - parasitology; Dermatitis, Allergic Contact - veterinary; Ectoparasitic Infestations - immunology; Ectoparasitic Infestations - parasitology; Ectoparasitic Infestations - veterinary; Felis domesticus; Female; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Hypersensitivity - diagnosis; Hypersensitivity - parasitology; Hypersensitivity - veterinary; Insect Bites and Stings - immunology; Insect Bites and Stings - veterinary; Insecta; Intradermal Tests - veterinary; Invertebrates; Predictive Value of Tests; Siphonaptera - immunology
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-006-0163-6

The results of intradermal testing with three commercial flea antigens and a serological test for IgE antibodies to flea antigens were compared with live flea challenge in cats. Eight control cats with no prior flea exposure had negative serological test and flea challenge results. By contrast, 17 out of 27 cats with previous flea exposure showed immediate reactivity to flea challenge; reactivity at 6, 24 and 48 h after flea exposure was noted in 12, 16 and 21 cats, respectively. Seventeen of these cats had positive serological test results. Seven cats showed immediate intradermal test reactions to the ARTU allergen, six reacted to the Biophady allergen, and six reacted to the Greer allergen. Intradermal test reactivity was less frequent at the other time points. Using the results of the flea challenge as the 'gold standard' for the presence or absence of sensitisation to fleas, the sensitivity and specificity of the serological test was 0.88 and 0.77, respectively. Sensitivities of the intradermal tests at the four time points ranged from 0 to 0.33, whereas the specificities ranged from 0.78 to 1.0. Live flea challenge is better able to detect cats with hypersensitivity to fleas than either intradermal or serological testing.