Thin frozen film method for visualization of storage proteins in mature rice grains

There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quali...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-10, Vol.72 (10), p.2779-2781
Main Authors: Saito, Y.(Kyoto Prefectural Univ. (Japan)), Nakatsuka, N, Shigemitsu, T, Tanaka, K, Morita, S, Satoh, S, Masumura, T
Format: Article
Language:English
Subjects:
ARROZ
Biological and medical sciences
ENDOSPERM
ENDOSPERMA
ENDOSPERME
Frozen Sections - methods
Fundamental and applied biological sciences. Psychology
IMMUNOFLUORESCENCE
immunofluorescence microscopy
INMUNOFLUORESCENCIA
MICROSCOPIA
MICROSCOPIE
MICROSCOPY
Oryza - growth & development
Oryza sativa
Plant Proteins
prolamin
PROLAMINAS
PROLAMINE
PROLAMINES
PROTEINAS DE RESERVA
PROTEINE DE RESERVE
RICE
rice grain
RIZ
storage protein
STORAGE PROTEINS
thin fresh-frozen section
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Description
Summary:There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.80337