Phenotypic changes and DNA methylation status in cryopreserved seeds of rye (Secale cereale L.)

Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed in the NLGRP: storage in a −18 °C freezer (conv...

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Bibliographic Details
Published in:Cryobiology 2018-06, Vol.82, p.8-14
Main Authors: Lu, Jie, Greene, Stephanie, Reid, Scott, Cruz, Von Mark V., Dierig, David A., Byrne, Patrick
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Cryopreservation
Cryopreservation - methods
DNA Methylation - genetics
Epigenetics
Genebank
Germination
metAFLP
Nitrogen - pharmacology
Orthodox seeds
Phenotype
Plant Roots - growth & development
Plants
Secale - genetics
Secale - growth & development
Seedlings - growth & development
Seeds - growth & development
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Summary:Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed in the NLGRP: storage in a −18 °C freezer (conventional storage) and storage in liquid nitrogen vapor phase at −135 to −180 °C (cryopreservation). To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. In laboratory evaluations of winter habit rye, seeds from cryopreserved samples had significantly higher normal germination percentage (P 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2018.04.015