Phenotypic changes and DNA methylation status in cryopreserved seeds of rye (Secale cereale L.)

Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed in the NLGRP: storage in a −18 °C freezer (conv...

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Published in:Cryobiology 2018-06, Vol.82, p.8-14
Main Authors: Lu, Jie, Greene, Stephanie, Reid, Scott, Cruz, Von Mark V., Dierig, David A., Byrne, Patrick
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Cryopreservation
Cryopreservation - methods
DNA Methylation - genetics
Epigenetics
Genebank
Germination
metAFLP
Nitrogen - pharmacology
Orthodox seeds
Phenotype
Plant Roots - growth & development
Plants
Secale - genetics
Secale - growth & development
Seedlings - growth & development
Seeds - growth & development
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container_title Cryobiology
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creator Lu, Jie
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description Conserving genetic diversity is a major priority of the National Laboratory for Genetic Resources Preservation (NLGRP), operated by the U.S. Department of Agriculture, Agricultural Research Service. There are two long-term preservation methods employed in the NLGRP: storage in a −18 °C freezer (conventional storage) and storage in liquid nitrogen vapor phase at −135 to −180 °C (cryopreservation). To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. In laboratory evaluations of winter habit rye, seeds from cryopreserved samples had significantly higher normal germination percentage (P 
doi_str_mv 10.1016/j.cryobiol.2018.04.015
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There are two long-term preservation methods employed in the NLGRP: storage in a −18 °C freezer (conventional storage) and storage in liquid nitrogen vapor phase at −135 to −180 °C (cryopreservation). To test the phenotypic and epigenetic effects of long-term cryopreservation of orthodox seeds, we evaluated 40 cereal rye accessions (20 spring habit and 20 winter habit) stored for 25 years under both conventional storage and cryogenic conditions. In laboratory evaluations of winter habit rye, seeds from cryopreserved samples had significantly higher normal germination percentage (P &lt; 0.05) and lower abnormal germination percentage (P &lt; 0.05) than those stored under conventional conditions. Cryopreserved spring habit rye also had higher normal germination percentage (P &lt; 0.01) than conventionally stored samples. In addition, winter rye seedlings from cryopreserved seeds had longer roots and smaller root diameter (P &lt; 0.05) than seedlings from conventionally stored seeds. In field evaluations conducted in Fort Collins, Colorado in 2014-15, spikes of plants grown from conventionally stored seeds of the winter accessions were slightly longer than those from cryopreserved seeds (P = 0.045). To detect DNA methylation changes, a methylation-sensitive amplified fragment length polymorphism (metAFLP) technique was applied to two accessions. After false discovery rate adjustment, no differences in methylation were detected between storage treatments on an individual locus basis. 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subjects Amplified Fragment Length Polymorphism Analysis
Cryopreservation
Cryopreservation - methods
DNA Methylation - genetics
Epigenetics
Genebank
Germination
metAFLP
Nitrogen - pharmacology
Orthodox seeds
Phenotype
Plant Roots - growth & development
Plants
Secale - genetics
Secale - growth & development
Seedlings - growth & development
Seeds - growth & development
title Phenotypic changes and DNA methylation status in cryopreserved seeds of rye (Secale cereale L.)
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