Isolation and characterization of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase gene from Botryococcus braunii, race B

The B race of a green microalga Botryococcus braunii Kützing produces triterpene hydrocarbons that is a promising source for biofuel. In this algal race, precursors of triterpene hydrocarbons are provided from the 2- C -methyl- d -erythritol 4-phosphate (MEP) pathway. The terminal enzyme of this pat...

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Bibliographic Details
Published in:Journal of plant research 2018-09, Vol.131 (5), p.839-848
Main Authors: Uchida, Hidenobu, Sumimoto, Koremitsu, Oki, Tomoka, Nishii, Ichiro, Mizohata, Eiichi, Matsunaga, Shigeki, Okada, Shigeru
Format: Article
Language:English
Subjects:
Algae
Amino acid sequence
Amino acids
Auxotrophy
Biofuels
Biomedical and Life Sciences
Biosynthesis
Botryococcus braunii
Deficient mutant
E coli
Erythritol
Hydrocarbons
Inoculation
Introns
Life Sciences
Liquid culture
Phylogeny
Plant Biochemistry
Plant Ecology
Plant Physiology
Plant Sciences
Proteins
Reductase
Regular Paper
Substrates
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Summary:The B race of a green microalga Botryococcus braunii Kützing produces triterpene hydrocarbons that is a promising source for biofuel. In this algal race, precursors of triterpene hydrocarbons are provided from the 2- C -methyl- d -erythritol 4-phosphate (MEP) pathway. The terminal enzyme of this pathway, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) is regarded as one of the key enzymes that affect yields of products in terpene biosynthesis. In order to better understand the MEP pathway of the alga, cDNA and genomic clones of HDR were obtained from B. braunii Showa strain. B. braunii HDR ( BbHDR ) is encoded on a single copy gene including a 1509-bp open reading frame that was intervened by 6 introns. The exon–intron structure of BbHDR genes did not show clear relation to phylogeny, while its amino acid sequence reflected phyla and classes well. BbHDR sequence was distinctive from that of the HDR protein from Escherichia coli in the residues involved in hydrogen-bond network that surrounds substrate. Introduction of BbHDR cDNA into an E. coli HDR deficient mutant resulted in recovery of its auxotrophy. BbHDR expression level was upregulated from the onset of liquid culture to the 24th day after inoculation with a 2.5-fold increase and retained its level in the subsequent period.
ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-018-1039-4