Screening of Serum Biomarkers and Establishment of a Decision Tree in Silica-Exposed Populations by Surface-Enhanced Laser Desorption Ionization Time-of-Fly Mass Spectrometry

Objective: To screen for serum biomarkers of silicosis and to study their roles using surface-enhanced laser desorption ionization time-offly mass spectrometry (SELDI-TOF-MS) techniques. Methods: The serum protein/peptide profile on the CM 10 proteinchip was acquired using SELDI-TOF-MS from workers...

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Bibliographic Details
Published in:Journal of occupational and environmental medicine 2007-07, Vol.49 (7), p.764-770
Main Authors: Wang, Shi-Xin, Zhao, Xue-Feng, Wei, Mao-Ti, Wu, You, Zeng, Jia-Wei, Liang, Xian-Cai, Yi, Jian-Hua, He, Bing, Tu, Zi-Guang
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers - blood
Chemical and industrial products toxicology. Toxic occupational diseases
China
Decision Trees
Desorption
Discriminant Analysis
Human exposure
Humans
Inorganic dusts (pneumoconiosises) and organic dusts (byssinosis etc.)
Male
Mass Screening
Medical sciences
Miscellaneous
ORIGINAL ARTICLES
Peptides - analysis
Peptides - blood
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Scientific imaging
Silica
Silicosis - blood
Silicosis - diagnosis
Silicosis - physiopathology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Studies
Toxicology
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Summary:Objective: To screen for serum biomarkers of silicosis and to study their roles using surface-enhanced laser desorption ionization time-offly mass spectrometry (SELDI-TOF-MS) techniques. Methods: The serum protein/peptide profile on the CM 10 proteinchip was acquired using SELDI-TOF-MS from workers classified by the Chinese national diagnostic standard for pneumoconiosis. Discriminant analysis was performed to establish a decision tree using protein/peptide peaks. Results: Protein/peptide peaks changed much more in silica-exposed populations than they did in the control. Discriminant analysis using Wilks' lambda method could give high sensitivity and specificity in distinguishing the silicaexposed population from the control; the decision tree could give 100% sensitivity and 95 % specificity in distinguishing the silicosis phase among the control group. Conclusions: Special proteins/peptides may change in silica-exposed workers and these changes may be used to distinguish silica-exposed populations from the control with the proper discriminant analytical method.
ISSN:1076-2752
1536-5948
DOI:10.1097/JOM.0b013e318058aa16