Cellular Uptake Mechanism of an Inorganic Nanovehicle and Its Drug Conjugates:  Enhanced Efficacy Due To Clathrin-Mediated Endocytosis

We present the mechanism for the cellular uptake of layered double hydroxide (LDH) nanoparticles that are internalized into MNNG/HOS cells principally via clathrin-mediated endocytosis. The intracellular LDHs are highly colocalized with not only typical endocytic proteins, such as clathrin heavy cha...

Full description

Saved in:
Bibliographic Details
Published in:Bioconjugate chemistry 2006-11, Vol.17 (6), p.1411-1417
Main Authors: Oh, Jae-Min, Choi, Soo-Jin, Kim, Sang-Tae, Choy, Jin-Ho
Format: Article
Language:English
Subjects:
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Antineoplastic Agents - toxicity
Cell Line
Cell Survival - drug effects
Cells
Chemical compounds
Chromatography, High Pressure Liquid
Clathrin - metabolism
Drugs
Endocytosis
G1 Phase - drug effects
Humans
Microscopy, Electron, Scanning
Nanoparticles
Nanostructures - ultrastructure
Proteins
X-Ray Diffraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We present the mechanism for the cellular uptake of layered double hydroxide (LDH) nanoparticles that are internalized into MNNG/HOS cells principally via clathrin-mediated endocytosis. The intracellular LDHs are highly colocalized with not only typical endocytic proteins, such as clathrin heavy chain, dynamin, and eps15, but also transferrin, a marker of the clathrin-mediated process, suggesting their specific internalization pathway. LDHs loaded with an anticancer drug (MTX−LDH) were also prepared to confirm the efficacy of LDHs as drug delivery systems. The cellular uptake of MTX was higher in MTX−LDH-treated cells than in MTX-treated cells, giving a lower IC50 value for MTX−LDH than for MTX only. The inhibition of the cell cycle was greater for MTX−LDH than for MTX only. This result clearly shows that the internalization of LDH nanoparticles via clathrin-mediated endocytosis may allow the efficient delivery of MTX−LDH in cells and thus enhance drug efficacy.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc0601323