A New Generic Enzyme Immunoassay for Sulfonamides

The study was directed to the development of generic immunoenzyme assay for sulfonamides. N-sulfanil-4-aminobutyric acid which mimics the common part of sulfonamides was used as immunogenic hapten. The obtained rabbit polyclonal antibodies allowed detection of the N-sulfanil-4-aminobutyric acid in i...

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Bibliographic Details
Published in:Analytical letters 2007-04, Vol.40 (6), p.1047-1062
Main Authors: Ermolenko, D. N., Eremin, S. A., Mart'ianov, A. A., Zherdev, A. V., Dzantiev, B. B.
Format: Article
Language:English
Subjects:
ELISA
generic immunoassay
N-sulfanil-4-aminobutyric acid
selectivity of antibodies
sulfanilamide
Sulfonamides
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Summary:The study was directed to the development of generic immunoenzyme assay for sulfonamides. N-sulfanil-4-aminobutyric acid which mimics the common part of sulfonamides was used as immunogenic hapten. The obtained rabbit polyclonal antibodies allowed detection of the N-sulfanil-4-aminobutyric acid in indirect competitive immunoenzyme assay down to 0.03 ng ml −1 . The lowest detection limit for the sulfonamides tested, 0.1 ng ml −1 , was observed for sulfamethoxypyridazine. Eleven other sulfonamides could be determined at concentrations ranging from 1-37 ng ml −1 . Thus, the proposed technique can be used in class-specific sulfonamides detection in products of animal origin.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710701296945