Bioelectrical Impedance Assay to Monitor Changes in Aspirin-Treated Human Colon Cancer HT-29 Cell Shape during Apoptosis

Physiological cell death occurs primarily through an evolutionarily conserved form of cell suicide termed apoptosis, which may be triggered by cytokines, depletion of growth factors, or certain chemicals. It is morphologically characterized by severe alterations in cell shape like cell shrinkage and...

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Bibliographic Details
Published in:Analytical letters 2007-02, Vol.40 (1), p.85-94
Main Authors: Yin, Hongying, Wang, Frank Lei, Wang, Angelo Lei, Cheng, Jing, Zhou, Yuxiang
Format: Article
Language:English
Subjects:
Analytical chemistry
apoptosis
Aspirin
bioelectrical impedance assay
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
ECIS
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Methods. Procedures. Technologies
Various methods and equipments
whole-cell biosensor
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Summary:Physiological cell death occurs primarily through an evolutionarily conserved form of cell suicide termed apoptosis, which may be triggered by cytokines, depletion of growth factors, or certain chemicals. It is morphologically characterized by severe alterations in cell shape like cell shrinkage and disintegration of cell-cell contacts. Aspirin and other nonsteroidal anti-inflammatory drugs (NSAIDs) inhibit proliferation of human colon cancer cells in vitro. We applied a noninvasive bioelectrical impedance assay referred to as electric cell-substrate impedance sensing (ECIS) in order to monitor the apoptosis-induced changes in human colon cancer HT-29 cell shape in an integral and quantitative fashion with a time resolution on the order of minutes. In whole-cell biosensors the cells are grown directly on the surface of small gold-film electrodes. From readings of the electrical impedance of the cell-covered electrode, we deduced alterations of aspirin on HT-29 cells in cell-cell and cell-substrate contacts. And the apoptosis was verified by transmission electron microscope.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710600952424