UHPLC-QqQ-MS/MS identification, quantification of polyphenols from Passiflora subpeltata fruit pulp and determination of nutritional, antioxidant, α-amylase and α-glucosidase key enzymes inhibition properties

• Published in: Food research international 2018-06, Vol.108, p.611-620
• Main Authors: Shanmugam, Saravanan, Gomes, Isla Alcântara, Denadai, Marina, dos Santos Lima, Bruno, de Souza Araújo, Adriano Antunes, Narain, Narendra, Neta, Maria Terezinha Santos Leite, Serafini, Mairim Russo, Quintans-Júnior, Lucindo José, Thangaraj, Parimelazhagan
• Format: Article
• Language: English
• Subjects: DPPH; Passiflora subpeltata; Phenolic compounds; UHPLC-QqQ-MS/MS; α-amylase; α-glucosidase
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2018.04.006

The diabetic key enzymes inhibition, nutritional, antioxidant activity and bioactive compounds identification of Passiflora subpeltata fruit pulp were investigated. Fifteen polyphenolic compounds including protocatechuic acid, ferulic acid, vanillic acid, epicatechin, p-coumaric acid, cinnamic acid, eriodictyol and quercetin-3-glucoside were identified in the pulp of this species by using UHPLC-QqQ-MS/MS analysis. The total carbohydrates and crude protein contents in fruit pulp were 2.62 mg glucose equivalent/g sample fruit pulp and 8.80 mg BSA equivalent/g sample fruit pulp, respectively. The fresh fruit pulp of P. subpeltata contained high total phenolic (724.76 mg GAE/g sample) content and it revealed very high DPPH• (IC50 of 5.667 μg/mL) and ABTS+• (6794.96 μM trolox equivalent/g sample) scavenging activities. In the key enzymes assays useful for diabetic inhibition the fresh fruit pulp characterized maximum inhibition of α-amylase and α-glucosidase IC50 of 18.69 and 32.63 μg/mL, respectively. Thus, these results lead to conclude that this fruit specie could be very useful source in nutraceutical products preparations for Type 2 diabetic suffering humans. [Display omitted] •15 phenolic compounds were identified in P. subpeltata fruit for the first time.•Different in vitro biological activities of P. subpeltata pulp were analyzed.•Protein and essential amino acids were detected in fruit pulp.•Fruit pulp serves as potent inhibitors of diabetic key enzymes.