When SUMO met splicing

Spliceosomal proteins have been revealed as SUMO conjugation targets. Moreover, we have reported that many of these are in a SUMO-conjugated form when bound to a pre-mRNA substrate during a splicing reaction. We demonstrated that SUMOylation of Prp3 (PRPF3), a component of the U4/U6 di-snRNP, is req...

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Bibliographic Details
Published in:RNA biology 2018-01, Vol.15 (6), p.689-695
Main Authors: Pozzi, Berta, Mammi, Pablo, Bragado, Laureano, Giono, Luciana E., Srebrow, Anabella
Format: Article
Language:English
Subjects:
Animals
Humans
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Point of View
post-translational modifications
Ribonucleoprotein, U4-U6 Small Nuclear - genetics
Ribonucleoprotein, U4-U6 Small Nuclear - metabolism
RNA Splicing - physiology
RNA Splicing Factors - genetics
RNA Splicing Factors - metabolism
spliceosome
Splicing
SR proteins
SRSF1
SUMO conjugation
SUMO-1 Protein - genetics
SUMO-1 Protein - metabolism
Sumoylation - physiology
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Summary:Spliceosomal proteins have been revealed as SUMO conjugation targets. Moreover, we have reported that many of these are in a SUMO-conjugated form when bound to a pre-mRNA substrate during a splicing reaction. We demonstrated that SUMOylation of Prp3 (PRPF3), a component of the U4/U6 di-snRNP, is required for U4/U6*U5 tri-snRNP formation and/or recruitment to active spliceosomes. Expanding upon our previous results, we have shown that the splicing factor SRSF1 stimulates SUMO conjugation to several spliceosomal proteins. Given the relevance of the splicing process, as well as the complex and dynamic nature of its governing machinery, the spliceosome, the molecular mechanisms that modulate its function represent an attractive topic of research. We posit that SUMO conjugation could represent a way of modulating spliceosome assembly and thus, splicing efficiency. How cycles of SUMOylation/de-SUMOylation of spliceosomal proteins become integrated throughout the highly choreographed spliceosomal cycle awaits further investigation.
ISSN:1547-6286
1555-8584
DOI:10.1080/15476286.2018.1457936