O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104

Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2018-05, Vol.83 (5), p.534-541
Main Authors: Zdorovenko, E. L., Wang, Y., Shashkov, A. S., Chen, T., Ovchinnikova, O. G., Liu, B., Golomidova, A. K., Babenko, V. V., Letarov, A. V., Knirel, Y. A.
Format: Article
Language:English
Subjects:
Antigens
Bacteria
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Biosynthesis
Carbohydrate Conformation
E coli
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - metabolism
Genomes
Glycerophosphate
Glycosylation
Health aspects
Life Sciences
Lipopolysaccharides
Magnetic resonance spectroscopy
Microbiology
Monosaccharides
NMR spectroscopy
O Antigens - chemistry
O Antigens - genetics
O Antigens - metabolism
Oligosaccharides
Polysaccharides
Saccharides
Two dimensional analysis
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Summary:Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1 H- and 13 C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297918050061