Effects of Low-Frequency Ultrasound on Microcystis aeruginosa from Cell Inactivation to Disruption

• Published in: Bulletin of environmental contamination and toxicology 2018-07, Vol.101 (1), p.117-123
• Main Authors: Tan, Xiao, Shu, Xiaoqian, Guo, Jiujia, Parajuli, Keshab, Zhang, Xidong, Duan, Zhipeng
• Format: Article
• Language: English
• Subjects: Algae; Algal control; Ammonia; Aquatic Pollution; Cavitation; Cell disruption; Chlorophyll; Chlorophyll A - metabolism; Cyanobacteria; Deactivation; Disruption; Earth and Environmental Science; Ecological effects; Ecotoxicology; Energy conservation; Environment; Environmental Chemistry; Environmental Health; Health problems; Inactivation; Microcystis; Microcystis - growth & development; Microcystis - metabolism; Microcystis aeruginosa; Models, Theoretical; Nitrogen; Nitrogen - analysis; Nutrients; Phosphorus; Phosphorus - analysis; Pollution; Public health; Soil Science & Conservation; Superoxide dismutase; Ultrasonic imaging; Ultrasonic Waves; Ultrasound; Waste Water Technology; Water Management; Water Pollutants, Chemical - analysis; Water Pollution Control
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-018-2348-y

Ultrasound can be used to induce cell resonance and cavitation to inhibit cyanobacterial growth, but it can also lead to increase in dissolved nutrients because of cell disruption. This study investigated the process from cell inactivation to disruption of Microcystis aeruginosa . Algal cells were sonicated (at 35 kHz) under various intensities and durations. Results showed that chlorophyll a content and F v / F m values decreased slightly within the first 5 min. Superoxide dismutase activity was stimulated and its peak value appeared at the fifth minute. After 20 min, considerable number of ruptured cells were observed and the concentrations of dissolved nitrogen and phosphorus increased rapidly. Finally, ammonia and nitrate merely composed a small portion of dissolved nitrogen. This study demonstrated that excessive ultrasound treatment can significantly rupture algal cells and lead to the release of cellular inclusions, which may cause ecological issues or public health problems. Based on our findings, ultrasonic intensity controlled at 0.035 W/mL and applied for a duration of 20 min delivers the optimal result in effectively inhibiting physiological activities of Microcystis aeruginosa without marked cell disruption. This will ultimately help to achieve algal control, while conserving energy and preserving the environment and human health.