MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells

MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-03, Vol.380 (2), p.205-210
Main Authors: Xia, Hongping, Qi, Yanting, Ng, Samuel S., Chen, Xiaona, Chen, Shen, Fang, Marong, Li, Dan, Zhao, Yu, Ge, Ruiguang, Li, Guo, Chen, Yangchao, He, Ming-Liang, Kung, Hsiang-fu, Lai, Lihui, Lin, Marie C.
Format: Article
Language:English
Subjects:
Brain - metabolism
Brain - pathology
Brain Neoplasms - genetics
Brain Neoplasms - pathology
CCNE1
Cell cycle
Cell Cycle - genetics
Cell Line, Tumor
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - pathology
Cyclins - genetics
Gene Expression Regulation, Neoplastic
Glioblastoma
Glioma - genetics
Glioma - pathology
Humans
MicroRNA
MicroRNAs - genetics
MicroRNAs - metabolism
MiR-15b
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Summary:MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between normal brain and glioma tissues from Chinese patients by miRNA microarray and identified a panel of differentially expressed miRNAs. Here, we studied the function of one miRNA, miR-15b, in glioma carcinogenesis and elucidated its downstream targets. Over-expression of miR-15b resulted in cell cycle arrest at G0/G1 phase while suppression of miR-15b expression resulted in a decrease of cell populations in G0/G1 and a corresponding increase of cell populations in S phase. We further showed that CCNE1 (encoding cyclin E1) is one of the downstream targets of miR-15b. Taken together, our findings indicate that miR-15b regulates cell cycle progression in glioma cells by targeting cell cycle-related molecules.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.12.169