Use of optical density as a measure of Claviceps africana conidial suspension concentration

Sorghum ergot, caused by Claviceps africana , has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research....

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Bibliographic Details
Published in:Australasian plant pathology 2006-01, Vol.35 (1), p.77-80
Main Authors: Herde, D. J., Ryley, M. J., Foster, S. D., Galea, V. J., Henzell, R. G., Jordan, D. R.
Format: Article
Language:English
Subjects:
Claviceps africana
colorimeter
inoculum concentration
light transmittance
Sorghum
Sorghum bicolor
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Summary:Sorghum ergot, caused by Claviceps africana , has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana , as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems.
ISSN:0815-3191
1448-6032
DOI:10.1071/AP05093