An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier

•This work developed a cheap, sensitive, portable and rapid-readout analytical system for clenbuterol.•Assay sensitivity was improved by the new probe and the amount of antibody reduced.•Bacteria (Escherichia coli) were introduced as signal carriers in the biosensor. Immunochromatographic assays (IC...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 2018-10, Vol.262, p.48-55
Main Authors: Huang, Qiong, Bu, Tong, Zhang, Wentao, Yan, Lingzhi, Zhang, Mengyue, Yang, Qingfeng, Huang, Lunjie, Yang, Baowei, Hu, Na, Suo, Yourui, Wang, Jianlong, Zhang, Daohong
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Antigens
Bacteria
Biosensing Techniques - methods
Chromatography, Affinity - instrumentation
Chromatography, Affinity - methods
Clenbuterol
Clenbuterol - analysis
Escherichia coli
Female
Gold - chemistry
Gold nanoparticles
Immunochromatographic assay
Limit of Detection
Metal Nanoparticles - chemistry
Mice, Inbred BALB C
Sensitivity and Specificity
Signal amplification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•This work developed a cheap, sensitive, portable and rapid-readout analytical system for clenbuterol.•Assay sensitivity was improved by the new probe and the amount of antibody reduced.•Bacteria (Escherichia coli) were introduced as signal carriers in the biosensor. Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2018.04.085