Metabolomics analysis of the potential anticancer mechanism of annonaceous acetogenins on a multidrug resistant mammary adenocarcinoma cell

Although annonaceous acetogenins (ACGs) have been reported to have antitumor activity for over three decades, and many of the underlying mechanism of ACGs on cancer have been clarified, there are still outstanding issues. In particular, the changes of small metabolite in cancer cells, caused by ACGs...

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Published in:Analytical biochemistry 2018-07, Vol.553, p.1-6
Main Authors: Ma, Chengyao, Li, Yue, Wu, Hanqing, Ji, Junyang, Sun, Qianqian, Song, Yilin, Wang, Shen, Li, Xiang, Chen, Yong, Chen, Jianwei
Format: Article
Language:English
Subjects:
Acetogenins - pharmacology
Adenocarcinoma - pathology
Animals
Annonaceous acetogenins
Arginine - metabolism
Cell Line, Tumor
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Glycerophospholipids - metabolism
Humans
Mammary adenocarcinoma
Mammary Neoplasms, Animal - pathology
Metabolomics
Multidrug resistance
Proline - metabolism
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Summary:Although annonaceous acetogenins (ACGs) have been reported to have antitumor activity for over three decades, and many of the underlying mechanism of ACGs on cancer have been clarified, there are still outstanding issues. In particular, the changes of small metabolite in cancer cells, caused by ACGs intake, have been reported rarely. Recent research has showed that cellular metabolic profiling coupled with ultra-flow liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UFLC-Q-TOF-MS) and multivariable statistical analysis enables a good understanding of ACGs' effects on multidrug resistant human mammary adenocarcinoma (MCF-7/Adr) cells. As a result, 23 potential biomarkers (p 1) were identified, and 5 pathways (impact-value > 0.10) identified. The differential metabolites suggested that ACGs affected metabolomics pathways, including arginine and proline metabolism, glycerophospholipid metabolism, taurine and hypotaurine metabolism, alanine, aspartate and glutamate metabolism and D-Glutamine and D-glutamate metabolism. [Display omitted] •A metabolomics approach by UFLC-Q-TOF-MS coupled with pattern recognition methods to ACGs for its antitumor activity.•Twenty three endogenous metabolites were identified and five metabolic pathways were found.•Barely visible differences in the metabolites between ACGs types were demonstrated.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2018.04.022