Chromatography of oxysterols

Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on g...

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Published in:Biochimie 2018-10, Vol.153, p.3-12
Main Authors: Dias, Irundika H.K., Wilson, Steven R., Roberg-Larsen, Hanne
Format: Article
Language:English
Subjects:
Chromatography
Chromatography, Gas - methods
Chromatography, Liquid - methods
Lipids
Oxysterols
Oxysterols - isolation & purification
Separation
Sterols
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description Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols. [Display omitted] •Gas chromatography for oxysterols is well established.•More diversity in liquid chromatography approaches.•Approaches to enhance speed and selectivity with LC are presented.•Few differences in chromatography between native and derivatized oxysterols.•Alternative approaches to separation of oxysterols exist, but are little explored.
doi_str_mv 10.1016/j.biochi.2018.05.004
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subjects Chromatography
Chromatography, Gas - methods
Chromatography, Liquid - methods
Lipids
Oxysterols
Oxysterols - isolation & purification
Separation
Sterols
title Chromatography of oxysterols
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