Study of solid-phase extraction for the determination of sequestering agents in river water by high-performance liquid chromatography

• Published in: Journal of Chromatography A 2009-02, Vol.1216 (7), p.1109-1114
• Main Authors: Kemmei, Tomoko, Kodama, Shuji, Muramoto, Tatsuya, Fujishima, Hironori, Yamamoto, Atsushi, Inoue, Yoshinori, Hayakawa, Kazuichi
• Format: Article
• Language: English
• Subjects: Analysis methods; Analytical chemistry; Applied sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Edetic Acid - analogs & derivatives; Edetic Acid - analysis; Egtazic Acid - analysis; Exact sciences and technology; Fresh Water - chemistry; Freshwater; High-performance liquid chromatography; Humans; Maps as Topic; Natural water pollution; Other chromatographic methods; Pentetic Acid - analysis; Pollution; River water; Sequestering agents; Sodium Compounds - chemistry; Solid Phase Extraction - methods; Solid-phase extraction; Sulfuric Acids - chemistry; Water Pollutants, Chemical - analysis; Water treatment and pollution
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2008.12.055

Anion-exchange solid-phase extraction accompanied with high-performance liquid chromatography has been developed for the determination of six kinds of aminopolycarboxylic acids (APCAs) in river water [N-(2-hydroxyethyl)ethylenediaminetriacetate (HEDTA), ethylenediaminetetraacetate (EDTA), 1,3-propanediaminetetraacetate (PDTA), diethylenetriaminepentaacetate (DTPA), 1,2-propanediaminetetraacetate (MeEDTA), and O,O′-bis(2-aminoethyl)ethyleneglycoltetraacetate (GEDTA)]. The enrichment of APCAs using an anion-exchange cartridge was successfully done by the removal of anions, which competed with APCAs in anion-exchange processes. Barium chloride solution was added to river water and the mixture was passed through On Guard II Ag and H cartridges and then a Bond Elut Jr.SAX cartridge to enrich APCAs. After elution, APCAs were analyzed on two reversed phase C30 columns connected in series and detected with ultraviolet detection. The enrichment using solid-phase extraction permitted the determination of APCAs in river water at concentrations as low as 1 nM. Good recoveries (83–111%) were obtained for each APCA by the standard addition method on three river water samples with high accuracy (RSD 1.8–9.5%). Applying this method, two kinds of APCAs, EDTA and DTPA, were determined in samples from the Oyabe and Senbo Rivers in Japan.