Label-free sensitive luminescence biosensor for immunoglobulin G based on Ag6Au6 ethisterone cluster-estrogen receptor α aggregation and graphene

• Published in: Talanta (Oxford) 2018-08, Vol.185, p.243-248
• Main Authors: Chen, Nannan, Guo, Wenjing, Lin, Zhixiang, Wei, Qiaohua, Chen, Guonan
• Format: Article
• Language: English
• Subjects: Ag6Au6 ethisterone cluster-estrogen receptor α aggregation; Graphene; Immunoglobulin G; Label-free luminescence biosensor
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.03.075

A specific and label-free “on-off-on” luminescence biosensor based on a novel heterometallic cluster [Ag6Au6(ethisterone)12]-estrogen receptor α (Ag6Au6Eth-ERα) aggregation utilizing graphene oxide (GO) as a quencher to lead a small background signal was firstly constructed to detect immunoglobulin G (IgG) with a simple process and high selectivity. The efficient photoluminescent (PL) Ag6Au6Eth-ERα aggregation is strongly quenched by GO. In the presence of IgG, the PL of this system will be restored, and perceivable by human eyes under UV lamp excitation (365 nm). The quenching mechanism of GO on Ag6Au6Eth-ERα and enhancement mechanism of IgG on Ag6Au6Eth-ERα-GO were investigated in detail. Under the optimum conditions, the biosensor for high sensitive IgG detection expressed a wider linear range of 0.0078–10 ng/mL and a lower detection limit of 0.65 pg/mL with good stability and repeatability, which provided a new approach for label-free IgG detection. [Display omitted] •A specific and label-free luminescence biosensor for ultrasensitive detection of IgG has been proposed by the combination of Ag6Au6Eth-ERα and GO with a simple process.•The luminescence brightness of the proposed biosensor for IgG detection could be perceivable by human eyes under 365 nm UV lamp excitation.•The present strategy definitely paves a way for the wide application of Ag6Au6Eth-ERα aggregation in biological field.