Proliferation and invasion of colon cancer cells are suppressed by knockdown of TOP2A

Recent research has shown that TOP2A plays an important role in the tumorigenesis of many malignancies, such as breast cancer, ovarian cancer, and prostate cancer. However, few studies have been conducted on TOP2A expression and functions in colon cancer. In the present study, we found that TOP2A ex...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2018-09, Vol.119 (9), p.7256-7263
Main Authors: Zhang, Rui, Xu, Jian, Zhao, Jian, Bai, Jing H.
Format: Article
Language:English
Subjects:
AKT protein
Annexin V
Apoptosis
BAX protein
Breast cancer
Cancer
Cell proliferation
Colon
Colon cancer
colon cancer cells
Colorectal cancer
invasion
Iodides
Ovarian cancer
Phosphorylation
proliferation
Propidium iodide
Prostate cancer
Proteins
Ribonucleic acid
RNA
siRNA
Tissues
TOP2A
Transfection
Tumorigenesis
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Summary:Recent research has shown that TOP2A plays an important role in the tumorigenesis of many malignancies, such as breast cancer, ovarian cancer, and prostate cancer. However, few studies have been conducted on TOP2A expression and functions in colon cancer. In the present study, we found that TOP2A expression was obviously elevated in colon cancer tissues compared to adjacent non‐cancerous tissues. Depletion of TOP2A in HCT116 and SW480 colon cancer cells by transfection of specific small interfering RNA significantly suppressed proliferation and inhibited invasion of cells, even induced apoptosis as indicated by both MTT assay, Annexin V/propidium iodide staining, and Transwell assay. Furthermore, we explored the underlying mechanisms. Knockdown of TOP2A not only affects the expression of cell apoptosis‐related (Bcl‐2 and Bax) and invasion‐related proteins (MMP‐2 and MMP‐9), but also reduced the phosphorylation levels of ERK and AKT. In conclusion, we showed that TOP2A was upregulated in colon cancer tissue samples and that TOP2A may serve as an oncogene in colon cancer. 1. Upregulation of TOP2A in colon cancer tissues, 2. Increased TOP2A expression in colon cancer is associated with tumor stage, and 3. Downregulation of TOP2A expression inhibits colon cancer cell proliferation.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.26916