Fast identification of dermatophytes by MALDI‐TOF/MS using direct transfer of fungal cells on ground steel target plates

• Published in: Mycoses 2018-09, Vol.61 (9), p.691-697
• Main Authors: Cunha, Keith C., Riat, Arnaud, Normand, Anne‐Cecile, Bosshard, Philipp P., Almeida, Margarete T. G., Piarroux, Renaud, Schrenzel, Jacques, Fontao, Lionel
• Format: Article
• Language: English
• Subjects: Clinical isolates; Dermatophytes; Ions; Libraries; MALDI‐TOF; Mass spectroscopy; Strains (organisms)
• ISSN: 0933-7407; 1439-0507
• DOI: 10.1111/myc.12793

Summary Dermatophytes cause human infections limited to keratinised tissues. We showed that the direct transfer method allows reliable identification of non‐dermatophytes mould and yeast by MALDI‐TOF/MS. We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/testing a panel of mass spectrum produced with strains genotypically identified and, 2/comparing MALDI‐TOF/MS identification to morphology‐based methods. Identification of dermatophytes using the Bruker library is poor. Our library provided 97% concordance between ITS sequencing and MALDI‐TOF/MS analysis with a panel of 1104 spectra corresponding to 276 strains. Direct transfer method using unpolished target plates allowed proper identification of 85% of dermatophytes clinical isolates most of which were common dermatophytes. A homemade dermatophyte MSP library is a prerequisite for accurate identification of species absent in the Bruker library but it also improves identification of species already listed in the database. The direct deposit method can be used to identify the most commonly found dermatophytes such as T. rubrum and T. interdigitale/mentagrophytes by MALDI‐TOF/MS.