Chronic exposure to low concentration of arsenic is immunotoxic to fish: Role of head kidney macrophages as biomarkers of arsenic toxicity to Clarias batrachus

• Published in: Aquatic toxicology 2009-04, Vol.92 (2), p.86-94
• Main Authors: Datta, Soma, Ghosh, Debabrata, Saha, Dhira Rani, Bhattacharaya, Shelley, Mazumder, Shibnath
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Aeromonas hydrophila - growth & development; Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Arsenic; Arsenic - toxicity; Biological and medical sciences; biomarkers; Biomarkers - analysis; Catfishes - growth & development; Catfishes - immunology; Catfishes - microbiology; Clarias batrachus; Dose-Response Relationship, Drug; Ecotoxicology, biological effects of pollution; fish; Fundamental and applied biological sciences. Psychology; General aspects; Head kidney; head kidney somatic index; Immunotoxicity; Interleukin-1beta - immunology; Kidney - drug effects; Kidney - immunology; Kidney - microbiology; Kidney - ultrastructure; kidneys; Macrophage; macrophages; Macrophages - drug effects; Macrophages - immunology; Macrophages - microbiology; Macrophages - ultrastructure; Microscopy, Electron, Transmission; Phagocytosis - drug effects; Phagocytosis - immunology; Reactive Oxygen Species - metabolism; Spectrophotometry, Atomic; Time Factors; toxicity testing; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Water Pollutants, Chemical - toxicity; water pollution
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2009.01.002

The present study was aimed at elucidating the effect of chronic low-level arsenic exposure on the head kidney (HK) of Clarias batrachus and at determining the changes in head kidney macrophage (HKM) activity in response to arsenic exposure. Chronic exposure (30 days) to arsenic (As 2O 3, 0.50 μM) led to significant increase in arsenic content in the HK accompanied by reduction in both HKM number and head kidney somatic index (HKSI). Arsenic induced HK hypertrophy, reduction in melano-macrophage population and increased hemosiderin accumulation. Transmission electron microscopy of 30 days exposed HKM revealed prominent endoplasmic reticulum, chromatin condensation and loss in structural integrity of nuclear membrane. Head kidney macrophages from exposed fish demonstrated significant levels of superoxide anions but on infection with Aeromonas hydrophila were unable to clear the intracellular bacteria and died. Exposure–challenge experiments with A. hydrophila revealed that chronic exposure to micromolar concentration of arsenic interfered with the phagocytic potential of HKM, helped in intracellular survival of the ingested bacteria inside the HKM inducing significant HKM cytotoxicity. The immunosuppressive effect of arsenic was further evident from the ability of A. hydrophila to colonize and disseminate efficiently in exposed fish. Enzyme linked immunosorbent assay indicated that chronic exposure to arsenic suppressed the production of pro-inflammatory ‘IL-1β like’ factors from HKM. It is concluded that arsenic even at very low concentration is immunotoxic to fish and the changes observed in HKM may provide a useful early biomarker of low-level xenobiotic exposure.