In vitro Direct Shoot Organogenesis and Regeneration of Plantlets from Leaf Explants of Sentang (Azadirachta excelsa)

A micropropagation protocol for the production of Azadirachta excelsa plantlets was developed through the formation of shoots using leaf cuttings. Leaf cuttings cultured on Murashige and Skoog (MS) medium supplemented with 2 mg L super(-1) benzylaminopurine (BAP), .2 mg L super(-1) kinetin and 6 mg...

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Bibliographic Details
Published in:Biotechnology (Faisalābād, Pakistan) Pakistan), 2006, Vol.5 (3), p.337-340
Main Authors: Foan, C C, Othman, R Y
Format: Article
Language:English
Subjects:
Azadirachta excelsa
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Summary:A micropropagation protocol for the production of Azadirachta excelsa plantlets was developed through the formation of shoots using leaf cuttings. Leaf cuttings cultured on Murashige and Skoog (MS) medium supplemented with 2 mg L super(-1) benzylaminopurine (BAP), .2 mg L super(-1) kinetin and 6 mg L super(-1) adenine sulphate produced an average of 5 shoot primordias per leaf cutting. Twenty five percent of these shoot primordias formed shoots with true leaves when cultured on MS medium supplemented with 1 mg L super(-1) BAP and 12.5 mg L super(-1) magnesium sulphate. One hundred percent of these shoots formed roots when transferred onto medium supplemented with 10 mg L super(-1) a-naphthaleneacetic acid (NAA).
ISSN:1682-296X
DOI:10.3923/biotech.2006.337.340