The incomplete substitution of lipopolysaccharide with O-chain prevents the establishment of effective symbiosis between Mesorhizobium loti NZP2213.1 and Lotus corniculatus

Mesorhizobium loti NZP2213.1 mutant obtained after random Tn 5 mutagenesis of M. loti NZP2213 was inefficient in nitrogen fixation on Lotus corniculatus. The transposon insertion was located within an ORF with a sequence similarity to a putative glycosyl transferase from Caulobacter crescentus. The...

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Bibliographic Details
Published in:Microbiological research 2009, Vol.164 (2), p.163-173
Main Authors: Turska-Szewczuk, Anna, Łotocka, Barbara, Kutkowska, Jolanta, Król, Jarosław, Urbanik-Sypniewska, Teresa, Russa, Ryszard
Format: Article
Language:English
Subjects:
6-Deoxytalose
Alphaproteobacteria - chemistry
Alphaproteobacteria - genetics
Alphaproteobacteria - physiology
Bacterial Proteins - genetics
Caulobacter crescentus
Deoxy Sugars - metabolism
Glycosyltransferases - genetics
Hexoses - metabolism
Lipopolysaccharide profiles
Lipopolysaccharides - chemistry
Lipopolysaccharides - metabolism
Loteae - microbiology
Loteae - physiology
Lotus
Lotus corniculatus
Mesorhizobium loti
Mutagenesis, Insertional
Root Nodules, Plant - microbiology
Root Nodules, Plant - physiology
Symbiosis
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Summary:Mesorhizobium loti NZP2213.1 mutant obtained after random Tn 5 mutagenesis of M. loti NZP2213 was inefficient in nitrogen fixation on Lotus corniculatus. The transposon insertion was located within an ORF with a sequence similarity to a putative glycosyl transferase from Caulobacter crescentus. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the mutant produced LPS of the same O-chain length but only half of the entire smooth LPS, compared to that of the parental strain. A greater diversity of the anomeric region as determined by NMR spectroscopy, reflected structural differences in the mutant repeating units represented by 6-deoxytalose, 2-OAc-6-deoxytalose, and 2-OMe-6-deoxytalose. In contrast to the completely O-acetylated 6-deoxytalose in wild-type OPS only partial O-acetylation was found in the mutant. The decrease of the LPS species with O-chains seems to be correlated with 6-deoxytalose deficiency. Microscopic examination of the nodules induced by the mutant revealed disturbances in infection thread development and premature senescence of symbiosomes. The impairment of mutant-induced symbiosomes to sustain latter stages of symbiosis could be a consequence of the decreased ratio of the hydrophobic to the hydrophilic LPSs.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2006.11.011