Effects of dietary microencapsulated Cymbopogon flexuosus essential oil on reproductive-related parameters in male Rhamdia quelen

• Published in: Fish physiology and biochemistry 2018-08, Vol.44 (4), p.1253-1264
• Main Authors: de Freitas Souza, Carine, Rampelotto, Cristine, Loureiro, Bruno Bianchi, Pereira, Fernanda Alves, Bianchini, Adriane Erbice, Corcini, Carine Dahl, Junior, Antonio Sergio Varela, Emanuelli, Tatiana, da Silva, Leila Picolli, da Costa, Sílvio Teixeira, Bertolin, Kalyne, Rovani, Monique Tomazele, Gonçalves, Paulo Bayard Dias, Heinzmann, Berta Maria, Baldisserotto, Bernardo
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Aquaculture; Biomedical and Life Sciences; Breeding stock; Catfish; Catfishes - physiology; Cymbopogon - chemistry; Cymbopogon flexuosus; Diet; Diet - veterinary; Dietary Supplements; DNA; Essential oils; Fish; Fish Proteins - metabolism; Freshwater & Marine Ecology; Freshwater fishes; Gene expression; Gonadosomatic index; Gonads; Histology; Histopathology; Life Sciences; Male; Males; Microcapsules; Morphology; Nucleotide sequence; Nutrition; Oil; Oils & fats; Oils, Volatile - pharmacology; Oxidative stress; Oxidative Stress - drug effects; Parameters; PCR; Pituitary; Pituitary gland; Plant Oils - pharmacology; Reproduction; Rhamdia quelen; Spermatozoa; Testosterone; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-018-0518-9

In aquaculture, nutrition and supplemented diets have been shown to affect broodstock reproductive performance. In this study, we investigated the effects of dietary supplementation with Cymbopogon flexuosus essential oil (CFEO) microcapsules on reproductive-related parameters in silver catfish ( Rhamdia quelen ) male broodfish. Adult male broodstocks were separated into three groups according to the concentrations of supplemented CFEO (0.0 = control; 1.0 or 3.0 mL per kg of diet). After 20 days under experimental conditions, the animals were euthanized and the gonads were harvested for gonadosomatic index, sperm analysis, oxidative stress, and histopathology; testosterone levels were measured in the plasma; gene expression of prl , smtl , pomca , and pomcb was assessed in the pituitary gland by real-time PCR. The results showed no alterations on reproductive parameters in R. quelen males treated with Cymbopogon flexuosus essential oil compared to the control-diet animals. In conclusion, CFEO microcapsules supplied for 20 days in the concentrations of 1.00 or 3.00 mL per kilogram of diet did not affect the reproduction criteria evaluated in this study in male silver catfish.