Comparative study on metabolite level in tissue-specific human mesenchymal stem cells by an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry

Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characterist...

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Bibliographic Details
Published in:Analytica chimica acta 2018-09, Vol.1024, p.112-122
Main Authors: Lee, Seul Ji, Yi, TacGhee, Ahn, Soo Hyun, Lim, Dong Kyu, Kim, Si-na, Lee, Hyun-Joo, Cho, Yun-Kyoung, Lim, Jae-Yol, Sung, Jong-Hyuk, Yun, Jeong-Ho, Lim, Johan, Song, Sun U., Kwon, Sung Won
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose-derived stem cell
Bone marrow
Bone marrow-derived stem cell
Chromatography
Comparative studies
Dental materials
Differentiation
Immunomodulation
Ligaments
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Mesenchymal stem cell
Mesenchymal stem cells
Mesenchyme
Metabolites
Metabolomics
Multivariate analysis
Periodontal ligament-derived stem cell
Periodontics
Quadrupoles
Salivary gland
Salivary gland-derived stem cell
Salivary glands
Stem cells
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Summary:Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characteristics, little is known about the biological differences between MSC populations derived from different tissues. In this study, we used MS to compare the endogenous metabolite level in the human MSCs originating from the bone marrow, adipose tissue, periodontal ligaments, and salivary glands. Using an optimized metabolomics technique, we verified that human MSCs exhibit differences in the endogenous metabolite level depending on their source material, while the multivariate analysis showed that 5 lysophosphatidylcholines and 3 lysophosphatidylethanolamines can serve as markers for the discrimination between MSC sources and may be related to differences in their differentiation capacity. These results may significantly contribute to further mechanistic studies on the MSCs and provide novel insights into the properties and optimal usage of MSCs from different tissues. [Display omitted] •Endogenous metabolite level of human mesenchymal stem cells (MSCs) was evaluated.•MSCs from different tissue sources were compared.•Metabolic markers to distinguish MSCs by source tissue were identified.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2018.04.018