Selenium bioaccumulation and associated nutraceutical properties in Calocybe indica mushroom cultivated on Se-enriched wheat straw

• Published in: Journal of bioscience and bioengineering 2018-10, Vol.126 (4), p.482-487
• Main Authors: Rathore, Himanshi, Sharma, Abhishek, Prasad, Shalinee, Sharma, Satyawati
• Format: Article
• Language: English
• Subjects: Agaricales - chemistry; Agaricales - growth & development; Agaricales - metabolism; Amino acids; Antioxidants; Antioxidants - analysis; Antioxidants - metabolism; Biomass yield; Calocybe indica; Culture Media - chemistry; Culture Media - metabolism; Dietary Supplements - analysis; Methanolic extracts; Plant Stems - chemistry; Plant Stems - microbiology; Polyphenols; Polyphenols - analysis; Polyphenols - metabolism; Polysaccharides - analysis; Polysaccharides - metabolism; Proteins; Selenium - analysis; Selenium - metabolism; Sodium selenite; Triticum - chemistry; Triticum - microbiology
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2018.04.010

Calocybe indica was cultivated on wheat straw enriched with various concentrations of sodium selenite (Na2SeO3), (2.5–40 μg/ml). The content of selenium (Se) in fruit bodies increased linearly by increasing the amount of Na2SeO3, although the biomass yield inhibited above 5 μg/ml. The fruit bodies accumulated inorganic Se into organic forms by integrating the Se into proteins (56%–68%), polysaccharides (22%–29%), and nucleic acids (1.4%–2.7%). Maximum protein content (25.31 g/100 g) was observed in fruit bodies harvested from the substrate enriched with 10 μg/ml Se. Amino acid profiling revealed the highest value of glutamic acid (4.73 g/100 g), followed by aspartic acid (1.80 g/100 g), and glycine (1.61 g/100 g) at 10 μg/ml. The Se enrichment also enhanced the total phenol, free radical 2,2-dipheynl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing power (FRAP) of methanolic extracts of fruiting bodies to almost double of their contents. The principle component analysis (PCA) illustrated close correlations amongst the biomass yield, polyphenols, and antioxidant activities at 5 μg/ml concentrations of the Se. •Edible mushroom Calocybe indica revealed a potential to bioaccumulate selenium.•Major portions of accumulated selenium were found to be associated with proteins.•Se-enriched C. indica fruiting bodies contained high protein and amino acid contents with very good antioxidant properties.•PCA illustrated the correlations amongst the contents of Se dosage, yield, proteins, phenols and antioxidant properties.