A device for high-throughput monitoring of degradation in soft tissue samples

This work describes the design and validation of a novel device, the High-Throughput Degradation Monitoring Device (HDD), for monitoring the degradation of 24 soft tissue samples over incubation periods of several days inside a cell culture incubator. The device quantifies sample degradation by moni...

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Bibliographic Details
Published in:Journal of biomechanics 2018-06, Vol.74, p.180-186
Main Authors: Tzeranis, D.S., Panagiotopoulos, I., Gkouma, S., Kanakaris, G., Georgiou, N., Vaindirlis, N., Vasileiou, G., Neidlin, M., Gkousioudi, A., Spitas, V., Macheras, G.A., Alexopoulos, L.G.
Format: Article
Language:English
Subjects:
Antibodies
Arthritis
Biomaterials
Biomechanics
Cartilage
Cartilage degeneration
Cell culture
Coefficient of variation
Collagen
Collagenase
Conflicts of interest
Cytokines
Deformation mechanisms
Degeneration
Degradation
Drug screening
Equilibrium
Extracellular matrix remodelling
Gravity
Growth factors
High throughput screening
Mechanical testing
Metabolism
Monitoring
Proteins
Sensors
Soft tissues
Thermal transients
Tissue engineering
Transplants & implants
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Description
Summary:This work describes the design and validation of a novel device, the High-Throughput Degradation Monitoring Device (HDD), for monitoring the degradation of 24 soft tissue samples over incubation periods of several days inside a cell culture incubator. The device quantifies sample degradation by monitoring its deformation induced by a static gravity load. Initial instrument design and experimental protocol development focused on quantifying cartilage degeneration. Characterization of measurement errors, caused mainly by thermal transients and by translating the instrument sensor, demonstrated that HDD can quantify sample degradation with
ISSN:0021-9290
1873-2380
DOI:10.1016/j.jbiomech.2018.04.040