Acute phase proteins, interleukin-6, tumor necrosis factor, nitric oxide and oxidative stress markers in horses with cutaneous habronemosis under field condition

• Published in: Veterinary parasitology 2018-05, Vol.255, p.20-25
• Main Authors: El-Deeb, W., Iacob, O., Fayez, M., Elgioushy, M., Shawaf, T., Ibrahim, A.
• Format: Article
• Language: English
• Subjects: Antioxidant; Cytokines; Habronema; Haptoglobin; Malondialdehyde
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2018.03.023

•Cutaneous habronemosis induce an acute phase response in infected horses.•The selected proinflammatory cytokines are higher in infected horses with habronemosis.•Cutaneous habronemosis induce a state of oxidative stress in examined horses.•Some biochemical markers could be considered to facilitate the diagnosis of cutaneous habronemosis.•Biomarkers could be used to monitor the progress of treatment. Habronemosis is a common parasitic disease of horses worldwide. In order to investigate how haptoglobin (Hp), serum amyloid A (SAA), oxidative stress markers, nitric oxide (NO), interleukin-6 (IL-6) and tumor necrosis factor (TNF- α), varies in cutaneous habronemosis, 30 horses with the clinical disease and 20 clinically healthy horses were included in the current study. The serum levels of Hp, SAA, and proinflammatory cytokines (IL-6 and TNF-α), NO, malondialdehyde (MDA), super oxide dismutase (SOD), glutathione (GSH), and total antioxidant capacity (TAC) were determined in horses before and after two weeks of treatment. The serum levels of Hp, SAA, IL-6, TNF-α and MDA were significantly elevated in infected horses as compared to the controls. Alternately, the serum levels of SOD, GSH, TAC and NO, were recorded low in infected horses as compared to the controls. All tested markers resumed the same levels after treatment as in control group. The Hp, SAA, IL-6, TNF-α, and MDA exhibited a high degree of clinical accuracy of the cases diagnosis. The area under the curve (AUC) for acute phase proteins (SAA, Hp), IL-6, TNF-α, and MDA was 0.87, 0.94, 0.96, 0.96 and 1.0, respectively. These findings showed that Hp, SAA, IL-6, TNF-α, and MDA may be supportive in the diagnosis of cutaneous habronemosis in horses and, simultaneously, they can also be used to monitor the progress of the treatment.