Development of a LC-MS method for simultaneous determination of amoxicillin and metronidazole in human serum using hydrophilic interaction chromatography (HILIC)

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2018-07, Vol.1089, p.78-83
• Main Authors: Kathriarachchi, Udani L., Vidhate, Sagar S., Al-Tannak, Naser, Thomson, Alison H., da Silva Neto, Michael J.J., Watson, David G.
• Format: Article
• Language: English
• Subjects: Antibiotic assay; Antibiotic concentrations; HILIC; LC-MS
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2018.05.012

A method was developed for the determination of amoxicillin and metronidazole in human serum. The procedure used was hydrophilic interaction chromatography (HILIC) followed by mass spectrometric (MS) detection. Chromatographic separation was achieved on a ZIC-HILIC column and the mobile phase consisted of a mixture of 0.1% (v/v) formic acid in water and 0.1% (v/v) formic acid in acetonitrile. The method was validated with regard to selectivity, accuracy, precision, calibration, lower limit of quantification (LOQ), extraction recovery and matrix effect. The LOQs were 0.0138 and 0.008 μg/ml for amoxicillin and metronidazole respectively, while for quantification purposes linearity was achieved in the range of 0.1 μg/ml to 6.4 μg/ml for both drugs with correlation coefficients >0.9990. The intraday precision (expressed as %RSD) and the accuracy (expressed as the % deviation from the nominal value) was 80%, while a considerable matrix effect (