Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I. Via cotyledonary nodes

• Published in: In vitro cellular & developmental biology. Plant 2007-05, Vol.43 (3), p.260-266
• Main Authors: Buendía-González, L., Orozco-Villafuerte, J., Cruz-Sosa, F., Chávez-Ávila, V. M., Vernon-Carter, E. J., Newton, R. J.
• Format: Article
• Language: English
• Subjects: 2,4-D; Acetic acid; adventitious roots; benzyladenine; Callus; Clonal propagation; Cotyledonary node explant; Domestication; explants; Fabaceae; Genetic diversity; Germination; Growth regulators; In vitro regeneration; indole acetic acid; indole butyric acid; Mesquite; MICROPROPAGATION; naphthaleneacetic acid; Overexploitation; Plant growth; Plant roots; Plantlets; Plants; Prosopis; Prosopis laevigata; Rooting; Scarification; Seedlings; Shoots; Trees; vermiculite; Vitamin C
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-007-9027-8

Plantlet regeneration in Prosopis laevigata (Humb. & Bonpl. ex Willd.) Johnston (Fabaceae), a multipurpose tree, has been achieved from cotyledonary nodes excised from in vitro grown seedlings. The explants were cultured on MS media containing different concentrations of N-6 benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-d) and a mixture of organic components. The highest number (3.37+0.51) of multiple shoots was observed in MS media containing 2,4-d (9.05 μM)+BA (6.62 μM). The regenerated shoots were then transferred onto half-strength MS medium containing a plant growth regulator that was either: indole-3-butyric acid, 1-naphthaleneacetic, indole-3-acetic acid, or 2,4-d as well as phytagel or vermiculite for adventitious root initiation. Best rooting efficiency of 44.0% was obtained when NAA (16.11 μM) and vermiculite were used. After rooting, the cloned plantlets were successfully hardened to ex vitro conditions. This work may help to reduce the devastation caused by the overexploitation of this species.