Derivatization method for the quantification of lactic acid in cell culture media via gas chromatography and applications in the study of cell glycometabolism

Lactic acid represents an important metabolite that reflects mitochondria function and may further serve as energy source for cancer cells. In light of this physiological and pathological significance, we developed a novel and sensitive gas chromatography method to detect lactic acid in cell culture...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2018-07, Vol.1090, p.1-6
Main Authors: Zhang, Hua-Yin, Zhang, Ping-Ping, Tan, Xiao-Xin, Wang, Ze-Ze, Lian, Kao-Qi, Xu, Xiang-Dong, Kang, Wei-Jun
Format: Article
Language:English
Subjects:
Alkyl chloroformate
Cell
Derivatization
Gas chromatography
Lactic acid
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Summary:Lactic acid represents an important metabolite that reflects mitochondria function and may further serve as energy source for cancer cells. In light of this physiological and pathological significance, we developed a novel and sensitive gas chromatography method to detect lactic acid in cell culture media. Here, ethyl chloroformate was selected as derivative reagent and the derivatization process was further optimized in terms of number of reagents and reaction time as well as extraction reagents. Under optimal conditions, good linearity was achieved in the tested calibration range. The limit of detection (LOD) was determined to be 0.67 μmol/L, the recovery rates were 99.6%–106% and the precision rate RSD was
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2018.05.015