Preparation and Properties of Nanoparticles, tRNA–Bivalent Metal Cation (Me2+) Complexes, and Prospects of Their Practical Use

The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca 2+ , Mn 2+ , Ni 2+ , Zn 2+ , Co 2+ , and Cu 2+ were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on t...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2018-03, Vol.479 (1), p.118-122
Main Authors: Danilevich, V. N., Sorokin, V. V., Moiseev, Ya. P., Sizova, S. V.
Format: Article
Language:English
Subjects:
Additives
Aptamers
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Calcium
Carbon dioxide
Cations
Cobalt
Copper
Life Sciences
Metal ions
Molecular Biology
Nanoparticles
Nuclease
Preservation
Reagents
Ribonucleic acid
RNA
Shelf life
siRNA
Thermal cycling
Transfection
tRNA
Zinc
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Summary:The patterns of formation of RNA nanoparticles (NPs) during thermal cycling of bacterial total tRNA in the presence of cations Ca 2+ , Mn 2+ , Ni 2+ , Zn 2+ , Co 2+ , and Cu 2+ were studied. The optimal conditions for the production of NPs were found, and it was revealed that their size depends on the ratio of the concentrations of Me 2+ and tRNA. The concentration of reagents for obtaining NPs of small size (from 5 to 100 nm) was selected. It was shown that tRNA-based nanoparticles can comprise short (20–50 nt) ribooligonucleotides, including aptamers and siRNAs. The stability of NPs during storage in buffer solutions of various composition was studied. It was found that the initial suspensions of NPs are quite stable, but they are rapidly destroyed in PBS buffer (pH 7.4). A simple and effective stabilizer (polyarginine) was found, the additives of which ensure the preservation of nanoparticles in PBS buffer for more than 5 h. Nanoparticles modified with the stabilizer are resistant to blood serum nucleases and can be used for transfection.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672918020187