Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume “Xue mei”

• Published in: In vitro cellular & developmental biology. Plant 2007-03, Vol.43 (2), p.95-100
• Main Authors: Ning, G. G., Bai, S. P., Bao, M. Z., Liu, L.
• Format: Article
• Language: English
• Subjects: Adventitious shoots; Aqueous solutions; benzyladenine; Cotyledons; Data analysis; Direct shoot regeneration; embryo culture; Embryos; explants; Forest regeneration; Gardens & gardening; Genetic diversity; Immature embryos; Immatures; indole butyric acid; naphthaleneacetic acid; Organogenesis; Plant cells; Plant growth; Plant roots; Plantlets; Plants; Prunus mume; Regeneration; RESEARCH REPORTS; Rooting; shoots; Studies; thidiazuron; Woody plants
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-007-9035-8

Using immature embryos and cotyledons as explants, a successful system to culture immature embryos and induce direct regeneration from cotyledons was established for Prunus mume “Xuemei”. For immature embryo culture, a high frequency of plantlet formation (89.5%) from the embryonic axis was obtained using half-strength Murashige and Skoog (1/2 MS) medium supplemented with 13.2 μM 6-benzyladenine (BA) and 2.7 μM 1-naphthaleneacetic (NAA). Shoots formed directly from cotyledons with the embryo axis intact when explants were cultured on 1/2 MS medium containing 2.2 μM BA with different combinations of NAA (2.7, 5.4 μM) and indole-3-butyric acid (IBA) (0, 2.5, 5.0 μM). Better results were achieved when the embryonic axis was removed from the cotyledons and cultured on 1/2 MS medium supplement with 13.2 μM BA, 2.7 μM NAA or 2.2 μM BA, 2.2 μM thidiazuron (TDZ), and 2.7 μM NAA, respectively. Regenerated shoots were successfully rooted on 1/2 MS or Woody Plant medium (WPM) supplemented with 2.5–5.0 μM IBA. The effect of the embryonic axis, BA, and TDZ on cotyledon regeneration was investigated in detail. Rooted plantlets were transferred to soil successfully.