High-frequency plant regeneration via adventitious shoot formation from deembryonated cotyledon explants of Sesamum indicum L

Sesamum indicum L. was used as an important oil crop in the world. An efficient protocol for in vitro plant regeneration via adventitious shoot formation from deem-bryonated cotyledon explants isolated from mature seeds of sesame is developed. Optimal medium for direct adventitious shoot formation w...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2007-05, Vol.43 (3), p.209-214
Main Authors: Seo, H. Y., Kim, Y. J., Park, T. I., Kim, H. S., Yun, S. J., Park, K. H., Oh, M. K., Choi, M. Y., Paik, C. H., Lee, Y. S., Choi, Y. E.
Format: Article
Language:English
Subjects:
Acetic acid
Adventitious shoot
Adventitious shoots
Cotyledons
Cultivars
DEVELOPMENTAL BIOLOGY/MORPHOGENESIS
Food products
Mosses
Naphthalene
Peat
Plant cells
Plant regeneration
Plantlets
Plants
Rooting
Seedlings
Seeds
Sesame
Sesamum indicum
Transgenic plants
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Summary:Sesamum indicum L. was used as an important oil crop in the world. An efficient protocol for in vitro plant regeneration via adventitious shoot formation from deem-bryonated cotyledon explants isolated from mature seeds of sesame is developed. Optimal medium for direct adventitious shoot formation was Murashige and Skoog (MS) medium with 22.2 μM 6-benzylaminopurin (BA) and 5.7 μM indole-3-acetic acid (IAA). Abscisic acid (3.8 μM ABA) and AgNO3 (29.4 μM) were effective in enhancing the frequency of adventitious shoot formation. Preculture of cotyledon explants on high sucrose concentration (6–9%) for 2 wk and subsequent transfer to 3% sucrose enhanced the frequency of adventitious shoot induction. Root formation from the adventitious shoots was easily achieved on MS medium containing 2.7 μM of α-naphthalene acetic acid (NAA). Regenerated plantlets were acclimatized on sand and peat moss (1:1), showing 95% survival with subsequent flowering and seed set. We established the high-frequency plant regeneration via adventitious shoot formation in S. indicum L.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-006-9017-2