Full-length and defective enterovirus G genomes with distinct torovirus protease insertions are highly prevalent on a Chinese pig farm

Recombination occurs frequently between enteroviruses (EVs) which are classified within the same species of the Picornaviridae family. Here, using viral metagenomics, the genomes of two recombinant EV-Gs (strains EVG 01/NC_CHI/2014 and EVG 02/NC_CHI/2014) found in the feces of pigs from a swine farm...

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Bibliographic Details
Published in:Archives of virology 2018-09, Vol.163 (9), p.2471-2476
Main Authors: Wang, Yan, Zhang, Wen, Liu, Zhijian, Fu, Xingli, Yuan, Jiaqi, Zhao, Jieji, Lin, Yuan, Shen, Quan, Wang, Xiaochun, Deng, Xutao, Delwart, Eric, Shan, Tongling, Yang, Shixing
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Capsid protein
Coronaviridae
Deletion mutant
Genomes
Hogs
Infectious Diseases
Insertion
Medical Microbiology
Papain
Proteinase
Recombinants
Recombination
Strains (organisms)
Virology
VP1 protein
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Summary:Recombination occurs frequently between enteroviruses (EVs) which are classified within the same species of the Picornaviridae family. Here, using viral metagenomics, the genomes of two recombinant EV-Gs (strains EVG 01/NC_CHI/2014 and EVG 02/NC_CHI/2014) found in the feces of pigs from a swine farm in China are described. The two strains are characterized by distinct insertion of a papain-like protease gene from toroviruses classified within the Coronaviridae family. According to recent reports the site of the torovirus protease insertion was located at the 2C/3A junction region in EVG 02/NC_CHI/2014. For the other variant EVG 01/NC_CHI/2014, the inserted protease sequence replaced the entire viral capsid protein region up to the VP1/2A junction. These two EV-G strains were highly prevalent in the same pig farm with all animals shedding the full-length genome (EVG 02/NC_CHI/2014) while 65% also shed the capsid deletion mutant (EVG 01/NC_CHI/2014). A helper-defective virus relationship between the two co-circulating EV-G recombinants is hypothesized.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-018-3875-x